| RRC ID |
583
|
| 著者 |
Kushiro T, Okamoto M, Nakabayashi K, Yamagishi K, Kitamura S, Asami T, Hirai N, Koshiba T, Kamiya Y, Nambara E.
|
| タイトル |
The Arabidopsis cytochrome P450 CYP707A encodes ABA 8'-hydroxylases: key enzymes in ABA catabolism.
|
| ジャーナル |
EMBO J
|
| Abstract |
The hormonal action of abscisic acid (ABA) in plants is controlled by the precise balance between its biosynthesis and catabolism. In plants, ABA 8'-hydroxylation is thought to play a predominant role in ABA catabolism. ABA 8'-hydroxylase was shown to be a cytochrome P450 (P450); however, its corresponding gene had not been identified. Through phylogenetic and DNA microarray analyses during seed imbibition, the candidate genes for this enzyme were narrowed down from 272 Arabidopsis P450 genes. These candidate genes were functionally expressed in yeast to reveal that members of the CYP707A family, CYP707A1-CYP707A4, encode ABA 8'-hydroxylases. Expression analyses revealed that CYP707A2 is responsible for the rapid decrease in ABA level during seed imbibition. During drought stress conditions, all CYP707A genes were upregulated, and upon rehydration a significant increase in mRNA level was observed. Consistent with the expression analyses, cyp707a2 mutants exhibited hyperdormancy in seeds and accumulated six-fold greater ABA content than wild type. These results demonstrate that CYP707A family genes play a major regulatory role in controlling the level of ABA in plants.
|
| 巻・号 |
23(7)
|
| ページ |
1647-56
|
| 公開日 |
2004-4-7
|
| DOI |
10.1038/sj.emboj.7600121
|
| PII |
7600121
|
| PMID |
15044947
|
| PMC |
PMC391058
|
| MeSH |
Abscisic Acid / chemistry
Abscisic Acid / metabolism*
Arabidopsis / enzymology*
Arabidopsis / genetics
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Cytochrome P-450 Enzyme System / genetics
Cytochrome P-450 Enzyme System / metabolism*
Gene Expression Profiling
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Genes, Plant
Mixed Function Oxygenases / genetics
Mixed Function Oxygenases / metabolism*
Molecular Structure
Oligonucleotide Array Sequence Analysis
Phenotype
Plant Proteins
Seeds / enzymology
|
| IF |
9.889
|
| 引用数 |
526
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pda05432 |
