論文 - 詳細
| RRC ID | 58368 |
|---|---|
| 著者 | Ryoden Y, Fujii T, Segawa K, Nagata S. |
| タイトル | Functional Expression of the P2X7 ATP Receptor Requires Eros. |
| ジャーナル | J Immunol |
| Abstract |
In response to extracellular ATP, the purinergic receptor P2X7 mediates various biological processes, including phosphatidylserine (PtdSer) exposure, phospholipid scrambling, dye uptake, ion transport, and IL-1β production. A genome-wide CRISPR screen for molecules responsible for ATP-induced PtdSer exposure identified a transmembrane protein, essential for reactive oxygen species (Eros), as a necessary component for P2X7 expression. An Eros-null mouse T cell line lost the ability to expose PtdSer, to scramble phospholipids, and to internalize a dye YO-PRO-1 and Ca2+ ions. Eros-null mutation abolished the ability of an LPS-primed human THP-1 macrophage cell line and mouse bone marrow-derived macrophages to secrete IL-1β in response to ATP. Eros is localized to the endoplasmic reticulum and functions as a chaperone for NADPH oxidase components. Similarly, Eros at the endoplasmic reticulum transiently associated with P2X7 to promote the formation of a stable homotrimeric complex of P2X7. These results indicated that Eros acts as a chaperone not only for NADPH oxidase, but also for P2X7, and contributes to the innate immune reaction. |
| 巻・号 | 204(3) |
| ページ | 559-568 |
| 公開日 | 2020-2-1 |
| DOI | 10.4049/jimmunol.1900448 |
| PII | jimmunol.1900448 |
| PMID | 31862710 |
| MeSH | Adenosine Triphosphate / metabolism Animals Calcium Signaling Gene Knockdown Techniques Humans Interleukin-1beta / metabolism Macrophages / immunology* Membrane Proteins / genetics Membrane Proteins / metabolism* Mice Mutation / genetics Phagocytosis / genetics Phosphatidylserines / metabolism Receptors, Purinergic P2X7 / genetics Receptors, Purinergic P2X7 / metabolism* THP-1 Cells |
| IF | 4.718 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pCMV-VSV-G-RSV-Rev (RDB04393) pCMV-VSV-G (RDB04392) |