RRC ID 5838
Author Herpin A, Nakamura S, Wagner TU, Tanaka M, Schartl M.
Title A highly conserved cis-regulatory motif directs differential gonadal synexpression of Dmrt1 transcripts during gonad development.
Journal Nucleic Acids Res.
Abstract Differential gene expression largely accounts for the coordinated manifestation of the genetic programme underlying embryonic development and cell differentiation. The 3' untranslated region (3'-UTR) of eukaryotic genes can contain motifs involved in regulation of gene expression at the post-transcriptional level. In the 3'-UTR of dmrt1, a key gene that functions in gonad development and differentiation, an 11-bp protein-binding motif was identified that mediates gonad-specific mRNA localization during embryonic and larval development of fish. Mutations that disrupt the 11-bp motif leading to in vitro protein-binding loss and selective transcript stabilization failure indicate a role for this motif in RNA stabilization through protein binding. The sequence motif was found to be conserved in most of the dmrt1 homologous genes from flies to humans suggesting a widespread conservation of this specific mechanism.
Volume 37(5)
Pages 1510-20
Published 2009-4
DOI 10.1093/nar/gkn1065
PII gkn1065
PMID 19139075
PMC PMC2655695
MeSH 3' Untranslated Regions / chemistry* Animals Cells, Cultured Fish Proteins / biosynthesis Fish Proteins / genetics* Gene Expression Regulation, Developmental* Gonads / embryology Gonads / growth & development Gonads / metabolism* Humans Mesoderm / metabolism Oryzias / embryology Oryzias / genetics Oryzias / growth & development Protein Biosynthesis RNA Stability RNA, Messenger / metabolism RNA-Binding Proteins / metabolism Regulatory Sequences, Ribonucleic Acid* Transcription Factors / biosynthesis Transcription Factors / genetics*
IF 11.147
Times Cited 11
Medaka Carbio strain (Cab)