論文 - 詳細
| RRC ID | 58571 |
|---|---|
| 著者 | Chatani M, Morimoto H, Takeyama K, Mantoku A, Tanigawa N, Kubota K, Suzuki H, Uchida S, Tanigaki F, Shirakawa M, Gusev O, Sychev V, Takano Y, Itoh T, Kudo A. |
| タイトル | Acute transcriptional up-regulation specific to osteoblasts/osteoclasts in medaka fish immediately after exposure to microgravity. |
| ジャーナル | Sci Rep |
| Abstract |
Bone loss is a serious problem in spaceflight; however, the initial action of microgravity has not been identified. To examine this action, we performed live-imaging of animals during a space mission followed by transcriptome analysis using medaka transgenic lines expressing osteoblast and osteoclast-specific promoter-driven GFP and DsRed. In live-imaging for osteoblasts, the intensity of osterix- or osteocalcin-DsRed fluorescence in pharyngeal bones was significantly enhanced 1 day after launch; and this enhancement continued for 8 or 5 days. In osteoclasts, the signals of TRAP-GFP and MMP9-DsRed were highly increased at days 4 and 6 after launch in flight. HiSeq from pharyngeal bones of juvenile fish at day 2 after launch showed up-regulation of 2 osteoblast- and 3 osteoclast- related genes. Gene ontology analysis for the whole-body showed that transcription of genes in the category "nucleus" was significantly enhanced; particularly, transcription-regulators were more up-regulated at day 2 than at day 6. Lastly, we identified 5 genes, c-fos, jun-B-like, pai-1, ddit4 and tsc22d3, which were up-regulated commonly in the whole-body at days 2 and 6, and in the pharyngeal bone at day 2. Our results suggested that exposure to microgravity immediately induced dynamic alteration of gene expression levels in osteoblasts and osteoclasts. |
| 巻・号 | 6 |
| ページ | 39545 |
| 公開日 | 2016-12-22 |
| DOI | 10.1038/srep39545 |
| PII | srep39545 |
| PMID | 28004797 |
| PMC | PMC5177882 |
| MeSH | Animals Animals, Genetically Modified Bone and Bones / metabolism Fishes Gene Expression Gene Expression Regulation* Luminescent Proteins / chemistry Oryzias / genetics Osteoblasts / cytology* Osteoblasts / metabolism Osteocalcin / metabolism Osteoclasts / cytology* Osteoclasts / metabolism Space Flight Transcription, Genetic* Transcriptome Up-Regulation Weightlessness* |
| IF | 3.998 |
| 引用数 | 11 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 96 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| メダカ | RANKL-GFP (TG1360) MMP9-DsRed (TG1357) osterix-DsRed/TRAP-GFP (TG1359) TRAP-DsRed (TG1362) |