| RRC ID |
58571
|
| 著者 |
Chatani M, Morimoto H, Takeyama K, Mantoku A, Tanigawa N, Kubota K, Suzuki H, Uchida S, Tanigaki F, Shirakawa M, Gusev O, Sychev V, Takano Y, Itoh T, Kudo A.
|
| タイトル |
Acute transcriptional up-regulation specific to osteoblasts/osteoclasts in medaka fish immediately after exposure to microgravity.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Bone loss is a serious problem in spaceflight; however, the initial action of microgravity has not been identified. To examine this action, we performed live-imaging of animals during a space mission followed by transcriptome analysis using medaka transgenic lines expressing osteoblast and osteoclast-specific promoter-driven GFP and DsRed. In live-imaging for osteoblasts, the intensity of osterix- or osteocalcin-DsRed fluorescence in pharyngeal bones was significantly enhanced 1 day after launch; and this enhancement continued for 8 or 5 days. In osteoclasts, the signals of TRAP-GFP and MMP9-DsRed were highly increased at days 4 and 6 after launch in flight. HiSeq from pharyngeal bones of juvenile fish at day 2 after launch showed up-regulation of 2 osteoblast- and 3 osteoclast- related genes. Gene ontology analysis for the whole-body showed that transcription of genes in the category "nucleus" was significantly enhanced; particularly, transcription-regulators were more up-regulated at day 2 than at day 6. Lastly, we identified 5 genes, c-fos, jun-B-like, pai-1, ddit4 and tsc22d3, which were up-regulated commonly in the whole-body at days 2 and 6, and in the pharyngeal bone at day 2. Our results suggested that exposure to microgravity immediately induced dynamic alteration of gene expression levels in osteoblasts and osteoclasts.
|
| 巻・号 |
6
|
| ページ |
39545
|
| 公開日 |
2016-12-22
|
| DOI |
10.1038/srep39545
|
| PII |
srep39545
|
| PMID |
28004797
|
| PMC |
PMC5177882
|
| MeSH |
Animals
Animals, Genetically Modified
Bone and Bones / metabolism
Fishes
Gene Expression
Gene Expression Regulation*
Luminescent Proteins / chemistry
Oryzias / genetics
Osteoblasts / cytology*
Osteoblasts / metabolism
Osteocalcin / metabolism
Osteoclasts / cytology*
Osteoclasts / metabolism
Space Flight
Transcription, Genetic*
Transcriptome
Up-Regulation
Weightlessness*
|
| IF |
3.998
|
| 引用数 |
11
|
| リソース情報 |
| メダカ |
RANKL-GFP (TG1360)
MMP9-DsRed (TG1357)
osterix-DsRed/TRAP-GFP (TG1359)
TRAP-DsRed (TG1362) |
