| RRC ID |
58658
|
| 著者 |
Ren T, Fan XX, Wang MF, Duan FG, Wei CL, Li RZ, Jiang ZB, Wang YW, Yao XJ, Chen MW, Tang YJ, Leung EL.
|
| タイトル |
miR‑20b promotes growth of non‑small cell lung cancer through a positive feedback loop of the Wnt/β‑catenin signaling pathway.
|
| ジャーナル |
Int J Oncol
|
| Abstract |
microRNAs (miRNAs or miRs) are endogenous noncoding single‑stranded RNA molecules that can regulate gene expression by targeting the 3'‑untranslated region and play an important role in many biological and pathological processes, such as inflammation and cancer. In this study, we found that miR‑20b was significantly increased in human non‑small cell lung cancer (NSCLC) cell lines and patient tissues, suggesting that it may possess a carcinogenic role in lung cancer. This miRNA promoted the proliferation, migration and invasion of NSCLC cells by targeting and downregulating the expression of adenomatous polyposis coli (APC), which is a negative regulator of the canonical Wnt signaling pathway. Wnt signaling activation may increase transcription of miR‑20b. Therefore, miR‑20b and canonical Wnt signaling were coupled through a feed‑forward positive feedback loop, forming a biological regulatory circuit. Finally, an in vivo investigation further demonstrated that an increase in miR‑20b promoted the growth of cancer cells. Overall, our findings offer evidence that miR‑20b may contribute to the development of NSCLC by inhibiting APC via the canonical Wnt signaling pathway.
|
| 巻・号 |
56(2)
|
| ページ |
470-479
|
| 公開日 |
2020-2-1
|
| DOI |
10.3892/ijo.2019.4940
|
| PMID |
31894264
|
| PMC |
PMC6959373
|
| MeSH |
Adenomatous Polyposis Coli Protein / genetics*
Aged
Animals
Carcinoma, Non-Small-Cell Lung / blood
Carcinoma, Non-Small-Cell Lung / genetics*
Carcinoma, Non-Small-Cell Lung / pathology
Carcinoma, Non-Small-Cell Lung / surgery
Case-Control Studies
Cell Line, Tumor
Cell Movement / genetics
Cell Proliferation / genetics
Feedback, Physiological
Female
Gene Expression Regulation, Neoplastic
Healthy Volunteers
Humans
Lung / pathology
Lung / surgery
Lung Neoplasms / blood
Lung Neoplasms / genetics*
Lung Neoplasms / pathology
Lung Neoplasms / surgery
Male
Mice
MicroRNAs / metabolism*
Middle Aged
Neoplasm Invasiveness / genetics
Pneumonectomy
Wnt Signaling Pathway / genetics*
Xenograft Model Antitumor Assays
|
| IF |
3.899
|
| 引用数 |
0
|
| リソース情報 |
| ヒト・動物細胞 |
PC-9(RCB4455) |
