| RRC ID |
58726
|
| 著者 |
Komatsu T, Shimizu T, Kanoh M, Miyakawa T, Satta Y, Yasukochi Y, Fujimoto R, Tada M, Machida K, Kataoka S, Udaka K.
|
| タイトル |
Development of a novel monoclonal antibody that binds to most HLA-A allomorphs in a conformation-dependent yet peptide-promiscuous fashion.
|
| ジャーナル |
Immunogenetics
|
| Abstract |
Specificity analyses of peptide binding to human leukocyte antigen (HLA)-A molecules have been hampered due to a lack of proper monoclonal antibodies (mAbs) for certain allomorphs, such as the prevalent HLA-A1 for Caucasians and HLA-A11 for Asians. We developed a mAb that recognizes a conformational epitope common to most HLA-A allomorphs. The mAb, named A-1, does not discriminate peptides by amino acid sequences, making it suitable for measuring peptide binding. A stabilization assay using TAP-deficient cell lines and A-1 was developed to investigate the specificity of peptide binding to HLA-A molecules. Regarding the evolution of HLA-A genes, the A-1 epitope has been conserved among most HLA-A allomorphs but was lost when the HLA-A gene diversified into the HLA-A*32, HLA-A*31, and HLA-A*33 lineages together with HLA-A*29 after bifurcating from the HLA-A*25 and HLA-A*26 branchs. The establishment of A-1 is expected to help researchers investigate the peptide repertoire and develop computational tools to identify cognate peptides. Since no HLA-A locus-specific mAb has been available, A-1 will also be useful for analyzing the locus-specific regulation of the HLA gene expression.
|
| 巻・号 |
72(3)
|
| ページ |
143-153
|
| 公開日 |
2020-4-1
|
| DOI |
10.1007/s00251-020-01154-w
|
| PII |
10.1007/s00251-020-01154-w
|
| PMID |
31970435
|
| MeSH |
Amino Acid Sequence
Antibodies, Monoclonal / immunology
Antibodies, Monoclonal / metabolism*
Cell Line, Tumor
Epitopes / immunology
HLA-A Antigens / chemistry
HLA-A Antigens / immunology*
HLA-A1 Antigen / chemistry
HLA-A1 Antigen / immunology*
Humans
Models, Molecular
Peptides / immunology
Protein Binding / immunology
Protein Conformation
|
| IF |
2.621
|
| 引用数 |
0
|
| リソース情報 |
| ヒト・動物細胞 |
HEV0400
HEV0012 |
