| RRC ID |
58730
|
| 著者 |
Wanna-Udom S, Terashima M, Lyu H, Ishimura A, Takino T, Sakari M, Tsukahara T, Suzuki T.
|
| タイトル |
The m6A methyltransferase METTL3 contributes to Transforming Growth Factor-beta-induced epithelial-mesenchymal transition of lung cancer cells through the regulation of JUNB.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
N6-Methyladenosine (m6A) is the most common internal chemical modification of mRNAs involved in many pathological processes including various cancers. In this study, we investigated the role of m6A methyltransferase METTL3 in TGF-β-induced epithelial-mesenchymal transition (EMT) of lung cancer cell lines. The expression of METTL3 and m6A RNA modification were increased during TGF-β-induced EMT of A549 and LC2/ad lung cancer cells. Knockdown of METTL3 inhibited TGF-β-induced morphological conversion of the cells, enhanced cell migration potential and the expression changes of EMT-related marker genes such as CDH1/E-cadherin, FN1/Fibronectin and VIM/Vimentin. Mechanistic investigations revealed that METTL3 knockdown decreased the m6A modification, total mRNA level and mRNA stability of JUNB, one of the important transcriptional regulators of EMT. Over-expression of JUNB partially rescued the inhibitory effects of METTL3 knockdown in the EMT phenotypes. This study demonstrates that m6A methyltransferase METTL3 is indispensable for TGF-β-induced EMT of lung cancer cells through the regulation of JUNB.
|
| 巻・号 |
524(1)
|
| ページ |
150-155
|
| 公開日 |
2020-3-26
|
| DOI |
10.1016/j.bbrc.2020.01.042
|
| PII |
S0006-291X(20)30105-4
|
| PMID |
31982139
|
| MeSH |
Cell Line, Tumor
Epithelial-Mesenchymal Transition / drug effects*
Epithelial-Mesenchymal Transition / genetics
Gene Expression Regulation, Neoplastic / drug effects
Gene Knockdown Techniques
Humans
Lung Neoplasms / genetics
Lung Neoplasms / metabolism*
Lung Neoplasms / pathology*
Methyltransferases / genetics
Methyltransferases / metabolism*
Phenotype
RNA Stability / drug effects
Transcription Factors / genetics
Transcription Factors / metabolism*
Transforming Growth Factor beta / pharmacology*
|
| IF |
2.985
|
| 引用数 |
5
|
| リソース情報 |
| ヒト・動物細胞 |
LC-2/ad(RCB0440)
A549 |
