| RRC ID |
58737
|
| Author |
Momoi Y, Nishikimi A, Du G, Kataoka T, Katagiri K.
|
| Title |
Phosphatidic acid regulates subcellular distribution of RA-GEFs critical for chemokine-dependent migration.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Integrin activation by Rap1-GTP is pivotal for lymphocyte trafficking. In this study, we show the phosphatidic acid (PA)-dependent membrane distribution of RA-GEF-1 and -2 (also known as Rapgef2 and 6), which are guanine nucleotide exchange factors for Rap1, plays important roles in lymphocyte migration. RA-GEF-1 associates with PA through 919-967 aa within CDC25 homology domain, and the deletion of this region of RA-GEF-1 inhibits chemokine-dependent migration. Chemokine stimulation induces temporal production of PA on the plasma membrane, which is not necessary for Rap1 activation, but the translocation of RA-GEFs. Thus, chemokine-dependent generation of PA is critical for lymphocyte migration through membrane localization of RA-GEFs.
|
| Volume |
524(2)
|
| Pages |
325-331
|
| Published |
2020-4-2
|
| DOI |
10.1016/j.bbrc.2020.01.080
|
| PII |
S0006-291X(20)30149-2
|
| PMID |
31996307
|
| MeSH |
Animals
Cell Line
Cell Movement*
Chemokines / metabolism*
Guanine Nucleotide Exchange Factors / metabolism*
HEK293 Cells
Humans
Lymphocytes / cytology
Lymphocytes / metabolism
Mice
Phosphatidic Acids / metabolism*
|
| IF |
2.985
|
| Times Cited |
1
|
| Resource |
| Human and Animal Cells |
293T(RCB2202) |
