RRC ID 58749
Author Iwahasi S, Rui F, Morine Y, Yamada S, Saito YU, Ikemoto T, Imura S, Shimada M.
Title Hepatic Stellate Cells Contribute to the Tumor Malignancy of Hepatocellular Carcinoma Through the IL-6 Pathway.
Journal Anticancer Res
Abstract BACKGROUND/AIM:The hepatic stellate cells (HSCs) have relationship to cancer progression. The aim of this study is to investigate the effect of HSCs and the role of IL-6/Stat3 pathway on hepatocellular carcinoma (HCC) progression.
MATERIALS AND METHODS:HCCs were co-cultured with HSCs. The viability and migration ability of cancer cells were detected. Epithelial-mesenchymal transition (EMT) marker (E-cadherin), stem cell marker (CD44) and p-signal transducer and activator of transcription 3 (p-STAT3) of cancer cells were evaluated. Finally, interleukin-6 (IL-6) neutralization was performed.
RESULTS:Co-culture of HCCs with HSCs increased cancer cell viability and migration ability. EMT and stemness of cancer cells increased with HSCs. Following IL-6 neutralization, phospho-STAT3 activation, cancer cell viability and migration, as well as EMT, and stemness of cancer cells decreased.
CONCLUSION:HSCs promoted HCC progression through the IL-6/STAT3 pathway.
Volume 40(2)
Pages 743-749
Published 2020-2
DOI 10.21873/anticanres.14005
PII 40/2/743
PMID 32014916
MeSH Carcinoma, Hepatocellular / metabolism* Carcinoma, Hepatocellular / pathology Cell Communication / physiology Cell Line, Tumor Cell Movement / physiology Cell Survival / physiology Coculture Techniques Hep G2 Cells Hepatic Stellate Cells / metabolism* Hepatic Stellate Cells / pathology Humans Interleukin-6 / metabolism* Liver Neoplasms / metabolism* Liver Neoplasms / pathology Neoplastic Stem Cells / metabolism Neoplastic Stem Cells / pathology STAT3 Transcription Factor / metabolism Signal Transduction
IF 1.935
Times Cited 0
Human and Animal Cells Hep G2, HuH-7