Reference - Detail
|Author||Iwahasi S, Rui F, Morine Y, Yamada S, Saito YU, Ikemoto T, Imura S, Shimada M.|
|Title||Hepatic Stellate Cells Contribute to the Tumor Malignancy of Hepatocellular Carcinoma Through the IL-6 Pathway.|
BACKGROUND/AIM:The hepatic stellate cells (HSCs) have relationship to cancer progression. The aim of this study is to investigate the effect of HSCs and the role of IL-6/Stat3 pathway on hepatocellular carcinoma (HCC) progression.
MATERIALS AND METHODS:HCCs were co-cultured with HSCs. The viability and migration ability of cancer cells were detected. Epithelial-mesenchymal transition (EMT) marker (E-cadherin), stem cell marker (CD44) and p-signal transducer and activator of transcription 3 (p-STAT3) of cancer cells were evaluated. Finally, interleukin-6 (IL-6) neutralization was performed.
RESULTS:Co-culture of HCCs with HSCs increased cancer cell viability and migration ability. EMT and stemness of cancer cells increased with HSCs. Following IL-6 neutralization, phospho-STAT3 activation, cancer cell viability and migration, as well as EMT, and stemness of cancer cells decreased.
CONCLUSION:HSCs promoted HCC progression through the IL-6/STAT3 pathway.
|MeSH||Carcinoma, Hepatocellular / metabolism* Carcinoma, Hepatocellular / pathology Cell Communication / physiology Cell Line, Tumor Cell Movement / physiology Cell Survival / physiology Coculture Techniques Hep G2 Cells Hepatic Stellate Cells / metabolism* Hepatic Stellate Cells / pathology Humans Interleukin-6 / metabolism* Liver Neoplasms / metabolism* Liver Neoplasms / pathology Neoplastic Stem Cells / metabolism Neoplastic Stem Cells / pathology STAT3 Transcription Factor / metabolism Signal Transduction|
|Human and Animal Cells||Hep G2, HuH-7|