RRC ID 58796
著者 Sasano Y, Fukumoto K, Tsukamoto Y, Akagi T, Akashi M.
タイトル Construction of 3D cardiac tissue with synchronous powerful beating using human cardiomyocytes from human iPS cells prepared by a convenient differentiation method.
ジャーナル J Biosci Bioeng
Abstract Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) as a new source of cardiac cells are expected to find use as tools in high-throughput screening for drug candidates and cardiotoxicity validation without the need for experimentation on animals. In recent years, it has been reported that drug screening using three-dimensional (3D) tissue is better than conventional 2D culture. Various methods have been developed for mass culture of hiPSC-CMs, and embryoid body (EB) formation is necessary in the majority of differentiation methods as this is reported to promote the differentiation of hiPSCs. However, these operations result in increased processing, cost and loss of hiPSCs. Here, we show alternative methods for differentiation to hiPSC-CMs from <100 μm hiPSC-clumps without EB formation and report on a 3D-tissue fabrication using hiPSC-CMs. The 3D cardiac tissue constructed by a layer-by-layer (LbL) cell coating technique (LbL-3D Heart) showed synchronous powerful beating. We conclude that this method enables cost-effective, reproducible and scalable hiPSC-CM production with high activity for tissue engineering, drug screening and regenerative medicine.
巻・号 129(6)
ページ 749-755
公開日 2020-6-1
DOI 10.1016/j.jbiosc.2020.01.001
PII S1389-1723(19)30925-9
PMID 32151485
MeSH Cell Culture Techniques Cell Differentiation* Cells, Cultured Embryoid Bodies Humans Induced Pluripotent Stem Cells / cytology* Myocytes, Cardiac / cytology* Tissue Engineering*
IF 2.366
リソース情報
ヒト・動物細胞 253G1(HPS0002)