論文 - 詳細
| RRC ID | 58826 |
|---|---|
| 著者 | Shibata S, Hayashi R, Kudo Y, Okubo T, Imaizumi T, Katayama T, Ishikawa Y, Kobayashi Y, Toga J, Taniguchi Y, Honma Y, Sekiguchi K, Nishida K. |
| タイトル | Cell-Type-Specific Adhesiveness and Proliferation Propensity on Laminin Isoforms Enable Purification of iPSC-Derived Corneal Epithelium. |
| ジャーナル | Stem Cell Reports |
| Abstract |
A treatment for intractable diseases is expected to be the replacement of damaged tissues with products from human induced pluripotent stem cells (hiPSCs). Target cell purification is a critical step for realizing hiPSC-based therapy. Here, we found that hiPSC-derived ocular cell types exhibited unique adhesion specificities and growth characteristics on distinct E8 fragments of laminin isoforms (LNE8s): hiPSC-derived corneal epithelial cells (iCECs) and other non-CECs rapidly adhered preferentially to LN332/411/511E8 and LN211E8, respectively, through differential expression of laminin-binding integrins. Furthermore, LN332E8 promoted epithelial cell proliferation but not that of the other eye-related cells, leading to non-CEC elimination by cell competition. Combining these features with magnetic sorting, highly pure iCEC sheets were fabricated. Thus, we established a simple method for isolating iCECs from various hiPSC-derived cells without using fluorescence-activated cell sorting. This study will facilitate efficient manufacture of iCEC sheets for corneal disease treatment and provide insights into target cell-specific scaffold selection. |
| 巻・号 | 14(4) |
| ページ | 663-676 |
| 公開日 | 2020-4-14 |
| DOI | 10.1016/j.stemcr.2020.02.008 |
| PII | S2213-6711(20)30064-3 |
| PMID | 32197114 |
| PMC | PMC7160305 |
| MeSH | Cell Adhesion / drug effects Cell Differentiation / drug effects Cell Proliferation / drug effects Cell Separation / methods* Cells, Cultured Epithelial Cells / cytology Epithelial Cells / drug effects Epithelial Cells / metabolism Epithelium, Corneal / cytology* Humans Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / drug effects Integrins / metabolism Laminin / pharmacology* Protein Isoforms / pharmacology |
| IF | 6.032 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) |