RRC ID 59002
著者 Lien PTK, Viet NTM, Mizuno T, Suda Y, Irie K.
タイトル Pop2 phosphorylation at S39 contributes to the glucose repression of stress response genes, HSP12 and HSP26.
ジャーナル PLoS One
Abstract The S. cerevisiae Pop2 protein is an exonuclease in the Ccr4-Not complex that is a conserved regulator of gene expression. Pop2 regulates gene expression post-transcriptionally by shortening the poly(A) tail of mRNA. A previous study has shown that Pop2 is phosphorylated at threonine 97 (T97) by Yak1 protein kinase in response to glucose limitation. However, the physiological importance of Pop2 phosphorylation remains unknown. In this study, we found that Pop2 is phosphorylated at serine 39 (S39) under unstressed conditions. The dephosphorylation of S39 was occurred rapidly after glucose depletion, and the addition of glucose to the glucose-deprived culture recovered this phosphorylation, suggesting that Pop2 phosphorylation at S39 is regulated by glucose. This glucose-regulated phosphorylation of Pop2 at S39 is dependent on Pho85 kinase. We previously reported that Pop2 takes a part in the cell wall integrity pathway by regulating LRG1 mRNA; however, S39 phosphorylation of Pop2 is not involved in LRG1 expression. On the other hand, Pop2 phosphorylation at S39 is involved in the expression of HSP12 and HSP26, which encode a small heat shock protein. In the medium supplemented with glucose, Pop2 might be phosphorylated at S39 by Pho85 kinase, and this phosphorylation contributes to repress the expression of HSP12 and HSP26. Glucose starvation inactivated Pho85, which resulted in the derepression of HSP12 and HSP26, together with other glucose sensing mechanisms. Our results suggest that Pho85-dependent phosphorylation of Pop2 is a part of the glucose sensing system in yeast.
巻・号 14(4)
ページ e0215064
公開日 2019-1-1
DOI 10.1371/journal.pone.0215064
PII PONE-D-18-29548
PMID 30973945
PMC PMC6459547
MeSH Catabolite Repression GTPase-Activating Proteins / genetics GTPase-Activating Proteins / metabolism Gene Expression Regulation, Fungal / drug effects* Glucose / pharmacology* Glycoproteins / genetics Glycoproteins / metabolism Heat-Shock Proteins / genetics Heat-Shock Proteins / metabolism* Phosphorylation Ribonucleases / genetics Ribonucleases / metabolism* Saccharomyces cerevisiae / drug effects Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* Serine / chemistry Serine / metabolism*
IF 2.74
引用数 1
リソース情報
酵母 BYP1419