RRC ID 59036
Author Tang Q, Rui M, Bu S, Wang Y, Chew LY, Yu F.
Title A microtubule polymerase is required for microtubule orientation and dendrite pruning in Drosophila.
Journal EMBO J
Abstract Drosophila class IV ddaC neurons selectively prune all larval dendrites to refine the nervous system during metamorphosis. During dendrite pruning, severing of proximal dendrites is preceded by local microtubule (MT) disassembly. Here, we identify an unexpected role of Mini spindles (Msps), a conserved MT polymerase, in governing dendrite pruning. Msps associates with another MT-associated protein TACC, and both stabilize each other in ddaC neurons. Moreover, Msps and TACC are required to orient minus-end-out MTs in dendrites. We further show that the functions of msps in dendritic MT orientation and dendrite pruning are antagonized by the kinesin-13 MT depolymerase Klp10A. Excessive MT depolymerization, which is induced by pharmacological treatment and katanin overexpression, also perturbs dendritic MT orientation and dendrite pruning, phenocopying msps mutants. Thus, we demonstrate that the MT polymerase Msps is required to form dendritic minus-end-out MTs and thereby promotes dendrite pruning in Drosophila sensory neurons.
Volume 39(10)
Pages e103549
Published 2020-5-18
DOI 10.15252/embj.2019103549
PMID 32267553
PMC PMC7232011
MeSH Animals Dendrites / physiology* Drosophila / genetics Drosophila / growth & development* Drosophila / metabolism Drosophila Proteins / genetics* Drosophila Proteins / metabolism* Gene Expression Regulation, Developmental Katanin / metabolism Kinesins / metabolism Microtubule-Associated Proteins / genetics* Microtubule-Associated Proteins / metabolism* Microtubules / metabolism Mutation Neuronal Plasticity
IF 9.889
Times Cited 0
Resource
Drosophila 5000R-3