論文 - 詳細
| RRC ID | 59036 |
|---|---|
| 著者 | Tang Q, Rui M, Bu S, Wang Y, Chew LY, Yu F. |
| タイトル | A microtubule polymerase is required for microtubule orientation and dendrite pruning in Drosophila. |
| ジャーナル | EMBO J |
| Abstract |
Drosophila class IV ddaC neurons selectively prune all larval dendrites to refine the nervous system during metamorphosis. During dendrite pruning, severing of proximal dendrites is preceded by local microtubule (MT) disassembly. Here, we identify an unexpected role of Mini spindles (Msps), a conserved MT polymerase, in governing dendrite pruning. Msps associates with another MT-associated protein TACC, and both stabilize each other in ddaC neurons. Moreover, Msps and TACC are required to orient minus-end-out MTs in dendrites. We further show that the functions of msps in dendritic MT orientation and dendrite pruning are antagonized by the kinesin-13 MT depolymerase Klp10A. Excessive MT depolymerization, which is induced by pharmacological treatment and katanin overexpression, also perturbs dendritic MT orientation and dendrite pruning, phenocopying msps mutants. Thus, we demonstrate that the MT polymerase Msps is required to form dendritic minus-end-out MTs and thereby promotes dendrite pruning in Drosophila sensory neurons. |
| 巻・号 | 39(10) |
| ページ | e103549 |
| 公開日 | 2020-5-18 |
| DOI | 10.15252/embj.2019103549 |
| PMID | 32267553 |
| PMC | PMC7232011 |
| MeSH | Animals Dendrites / physiology* Drosophila / genetics Drosophila / growth & development* Drosophila / metabolism Drosophila Proteins / genetics* Drosophila Proteins / metabolism* Gene Expression Regulation, Developmental Katanin / metabolism Kinesins / metabolism Microtubule-Associated Proteins / genetics* Microtubule-Associated Proteins / metabolism* Microtubules / metabolism Mutation Neuronal Plasticity |
| IF | 9.889 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 5000R-3 |