RRC ID 59042
Author Chougule A, Lapraz F, Földi I, Cerezo D, Mihály J, Noselli S.
Title The Drosophila actin nucleator DAAM is essential for left-right asymmetry.
Journal PLoS Genet
Abstract Left-Right (LR) asymmetry is essential for organ positioning, shape and function. Myosin 1D (Myo1D) has emerged as an evolutionary conserved chirality determinant in both Drosophila and vertebrates. However, the molecular interplay between Myo1D and the actin cytoskeleton underlying symmetry breaking remains poorly understood. To address this question, we performed a dual genetic screen to identify new cytoskeletal factors involved in LR asymmetry. We identified the conserved actin nucleator DAAM as an essential factor required for both dextral and sinistral development. In the absence of DAAM, organs lose their LR asymmetry, while its overexpression enhances Myo1D-induced de novo LR asymmetry. These results show that DAAM is a limiting, LR-specific actin nucleator connecting up Myo1D with a dedicated F-actin network important for symmetry breaking.
Volume 16(4)
Pages e1008758
Published 2020-4-1
DOI 10.1371/journal.pgen.1008758
PMID 32324733
PMC PMC7200016
MeSH Actin Cytoskeleton / metabolism Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism* Animals Body Patterning* Drosophila Drosophila Proteins / genetics Drosophila Proteins / metabolism* Myosins / genetics Myosins / metabolism
IF 5.175
Times Cited 1