RRC ID |
59042
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著者 |
Chougule A, Lapraz F, Földi I, Cerezo D, Mihály J, Noselli S.
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タイトル |
The Drosophila actin nucleator DAAM is essential for left-right asymmetry.
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ジャーナル |
PLoS Genet
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Abstract |
Left-Right (LR) asymmetry is essential for organ positioning, shape and function. Myosin 1D (Myo1D) has emerged as an evolutionary conserved chirality determinant in both Drosophila and vertebrates. However, the molecular interplay between Myo1D and the actin cytoskeleton underlying symmetry breaking remains poorly understood. To address this question, we performed a dual genetic screen to identify new cytoskeletal factors involved in LR asymmetry. We identified the conserved actin nucleator DAAM as an essential factor required for both dextral and sinistral development. In the absence of DAAM, organs lose their LR asymmetry, while its overexpression enhances Myo1D-induced de novo LR asymmetry. These results show that DAAM is a limiting, LR-specific actin nucleator connecting up Myo1D with a dedicated F-actin network important for symmetry breaking.
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巻・号 |
16(4)
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ページ |
e1008758
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公開日 |
2020-4-1
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DOI |
10.1371/journal.pgen.1008758
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PII |
PGENETICS-D-19-01054
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PMID |
32324733
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PMC |
PMC7200016
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MeSH |
Actin Cytoskeleton / metabolism
Adaptor Proteins, Signal Transducing / genetics
Adaptor Proteins, Signal Transducing / metabolism*
Animals
Body Patterning*
Drosophila
Drosophila Proteins / genetics
Drosophila Proteins / metabolism*
Myosins / genetics
Myosins / metabolism
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IF |
5.175
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引用数 |
1
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リソース情報 |
ショウジョウバエ |
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