論文 - 詳細
| RRC ID | 59085 |
|---|---|
| 著者 | Kashyap DR, Kowalczyk DA, Shan Y, Yang CK, Gupta D, Dziarski R. |
| タイトル | Formate dehydrogenase, ubiquinone, and cytochrome bd-I are required for peptidoglycan recognition protein-induced oxidative stress and killing in Escherichia coli. |
| ジャーナル | Sci Rep |
| Abstract |
Mammalian Peptidoglycan Recognition Proteins (PGRPs) kill bacteria through induction of synergistic oxidative, thiol, and metal stress. PGRPs induce oxidative stress in bacteria through a block in the respiratory chain, which results in decreased respiration and incomplete reduction of oxygen (O2) to hydrogen peroxide (H2O2). In this study we identify the site of PGRP-induced generation of H2O2 in Escherichia coli. Tn-seq screening of E. coli Tn10 insertion library revealed that mutants in formate dehydrogenase (FDH) genes had the highest survival following PGRP treatment. Mutants lacking functional FDH-O had abolished PGRP-induced H2O2 production and the highest resistance to PGRP-induced killing, and formate enhanced PGRP-induced killing and H2O2 production in an FDH-dependent manner. Mutants in ubiquinone synthesis (but not menaquinone and demethylmenaquinone) and cytochrome bd-I (but not cytochromes bo3 and bd-II) also had completely abolished PGRP-induced H2O2 production and high resistance to PGRP-induced killing. Because electrons in the respiratory chain flow from dehydrogenases' substrates through quinones and then cytochromes to O2, these results imply that the site of PGRP-induced incomplete reduction of O2 to H2O2 is downstream from dehydrogenases and ubiquinone at the level of cytochrome bd-I, which results in oxidative stress. These results reveal several essential steps in PGRP-induced bacterial killing. |
| 巻・号 | 10(1) |
| ページ | 1993 |
| 公開日 | 2020-2-6 |
| DOI | 10.1038/s41598-020-58302-1 |
| PII | 10.1038/s41598-020-58302-1 |
| PMID | 32029761 |
| PMC | PMC7005000 |
| MeSH | Animals Carrier Proteins / genetics Carrier Proteins / isolation & purification Carrier Proteins / metabolism* Cell Line Cytochrome d Group / biosynthesis Cytochromes b / biosynthesis Drosophila melanogaster Escherichia coli / physiology* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Formate Dehydrogenases / genetics Formate Dehydrogenases / metabolism* Host Microbial Interactions* Humans Hydrogen Peroxide / metabolism Mutation Oxidation-Reduction Oxidative Stress / physiology Recombinant Proteins / genetics Recombinant Proteins / isolation & purification Recombinant Proteins / metabolism Ubiquinone / biosynthesis |
| IF | 4.011 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | Keio collection |