RRC ID 59125
著者 Slaidina M, Banisch TU, Gupta S, Lehmann R.
タイトル A single-cell atlas of the developing Drosophila ovary identifies follicle stem cell progenitors.
ジャーナル Genes Dev
Abstract Addressing the complexity of organogenesis at a system-wide level requires a complete understanding of adult cell types, their origin, and precursor relationships. The Drosophila ovary has been a model to study how coordinated stem cell units, germline, and somatic follicle stem cells maintain and renew an organ. However, lack of cell type-specific tools have limited our ability to study the origin of individual cell types and stem cell units. Here, we used a single-cell RNA sequencing approach to uncover all known cell types of the developing ovary, reveal transcriptional signatures, and identify cell type-specific markers for lineage tracing. Our study identifies a novel cell type corresponding to the elusive follicle stem cell precursors and predicts subtypes of known cell types. Altogether, we reveal a previously unanticipated complexity of the developing ovary and provide a comprehensive resource for the systematic analysis of ovary morphogenesis.
巻・号 34(3-4)
ページ 239-249
公開日 2020-2-1
DOI 10.1101/gad.330464.119
PII gad.330464.119
PMID 31919193
PMC PMC7000915
MeSH Animals Drosophila / cytology* Drosophila / genetics Drosophila / metabolism Female Models, Animal Ovarian Follicle / cytology* Ovary / cytology Sequence Analysis, RNA Single-Cell Analysis Stem Cells / cytology* Transcription, Genetic
IF 9.527
引用数 1
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