論文 - 詳細
| RRC ID | 59126 |
|---|---|
| 著者 | Ng CL, Qian Y, Schulz C. |
| タイトル | Notch and Delta are required for survival of the germline stem cell lineage in testes of Drosophila melanogaster. |
| ジャーナル | PLoS One |
| Abstract |
In all metazoan species, sperm is produced from germline stem cells. These self-renew and produce daughter cells that amplify and differentiate dependent on interactions with somatic support cells. In the male gonad of Drosophila melanogaster, the germline and somatic cyst cells co-differentiate as cysts, an arrangement in which the germline is completely enclosed by cytoplasmic extensions from the cyst cells. Notch is a developmentally relevant receptor in a pathway requiring immediate proximity with the signal sending cell. Here, we show that Notch is expressed in the cyst cells of wild-type testes. Notch becomes activated in the transition zone, an apical area of the testes in which the cyst cells express stage-specific transcription factors and the enclosed germline finalizes transit-amplifying divisions. Reducing the ligand Delta from the germline cells via RNA-Interference or reducing the receptor Notch from the cyst cells via CRISPR resulted in cell death concomitant with loss of germline cells from the transition zone. This shows that Notch signaling is essential for the survival of the germline stem cell lineage. |
| 巻・号 | 14(9) |
| ページ | e0222471 |
| 公開日 | 2019-1-1 |
| DOI | 10.1371/journal.pone.0222471 |
| PII | PONE-D-19-18655 |
| PMID | 31513679 |
| PMC | PMC6742463 |
| MeSH | Adult Germline Stem Cells / cytology Adult Germline Stem Cells / metabolism Animals Cell Differentiation Cell Lineage Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Germ Cells / metabolism* Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism* Male Membrane Proteins / genetics Membrane Proteins / metabolism* RNA Interference Receptors, Notch / genetics Receptors, Notch / metabolism* Signal Transduction Spermatozoa / metabolism Stem Cells / cytology Stem Cells / metabolism Testis / metabolism |
| IF | 2.74 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#104055 |