論文 - 詳細
| RRC ID | 59131 |
|---|---|
| 著者 | Lin T, Kao HH, Chou CH, Chou CY, Liao YC, Lee HH. |
| タイトル | Rab11 activation by Ik2 kinase is required for dendrite pruning in Drosophila sensory neurons. |
| ジャーナル | PLoS Genet |
| Abstract |
Neuronal pruning is a commonly observed phenomenon for the developing nervous systems to ensure precise wiring of neural circuits. The function of Ik2 kinase and its downstream mediator, Spindle-F (Spn-F), are essential for dendrite pruning of Drosophila sensory neurons during development. However, little is known about how Ik2/Spn-F signaling is transduced in neurons and ultimately results in dendrite pruning. Our genetic analyses and rescue experiments demonstrated that the small GTPase Rab11, especially the active GTP-bound form, is required for dendrite pruning. We also found that Rab11 shows genetic interactions with spn-F and ik2 on pruning. Live imaging of single neurons and antibody staining reveal normal Ik2 kinase activation in Rab11 mutant neurons, suggesting that Rab11 could have a functional connection downstream of and/or parallel to the Ik2 kinase signaling. Moreover, we provide biochemical evidence that both the Ik2 kinase activity and the formation of Ik2/Spn-F/Rab11 complexes are central to promote Rab11 activation in cells. Together, our studies reveal that a critical role of Ik2/Spn-F signaling in neuronal pruning is to promote Rab11 activation, which is crucial for dendrite pruning in neurons. |
| 巻・号 | 16(2) |
| ページ | e1008626 |
| 公開日 | 2020-2-1 |
| DOI | 10.1371/journal.pgen.1008626 |
| PII | PGENETICS-D-19-01222 |
| PMID | 32059017 |
| PMC | PMC7046344 |
| MeSH | Animals Animals, Genetically Modified Cell Line Dendrites / physiology Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development* Embryo, Nonmammalian Gene Expression Regulation, Developmental* Gene Knockdown Techniques I-kappa B Kinase / metabolism* Intravital Microscopy Microtubule-Associated Proteins / metabolism Neuronal Plasticity / genetics* Sensory Receptor Cells / cytology Sensory Receptor Cells / physiology* Signal Transduction / physiology Time-Lapse Imaging rab GTP-Binding Proteins / genetics rab GTP-Binding Proteins / metabolism* |
| IF | 5.175 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#109944 DGRC#109945 |