| RRC ID |
59156
|
| 著者 |
Yoong LF, Lim HK, Tran H, Lackner S, Zheng Z, Hong P, Moore AW.
|
| タイトル |
Atypical Myosin Tunes Dendrite Arbor Subdivision.
|
| ジャーナル |
Neuron
|
| Abstract |
Dendrite arbor pattern determines the functional characteristics of a neuron. It is founded on primary branch structure, defined through cell intrinsic and transcription-factor-encoded mechanisms. Developing arbors have extensive acentrosomal microtubule dynamics, and here, we report an unexpected role for the atypical actin motor Myo6 in creating primary branch structure by specifying the position, polarity, and targeting of these events. We carried out in vivo time-lapse imaging of Drosophila adult sensory neuron differentiation, integrating machine-learning-based quantification of arbor patterning with molecular-level tracking of cytoskeletal remodeling. This revealed that Myo6 and the transcription factor Knot regulate transient surges of microtubule polymerization at dendrite tips; they drive retrograde extension of an actin filament array that specifies anterograde microtubule polymerization and guides these microtubules to subdivide the tip into multiple branches. Primary branches delineate functional compartments; this tunable branching mechanism is key to define and diversify dendrite arbor compartmentalization.
|
| 巻・号 |
106(3)
|
| ページ |
452-467.e8
|
| 公開日 |
2020-5-6
|
| DOI |
10.1016/j.neuron.2020.02.002
|
| PII |
S0896-6273(20)30101-X
|
| PMID |
32155441
|
| MeSH |
Animals
Cell Line
Cells, Cultured
Dendrites / metabolism*
Dendrites / physiology
Drosophila Proteins / metabolism
Drosophila melanogaster
Microtubules / metabolism
Myosin Heavy Chains / genetics
Myosin Heavy Chains / metabolism*
Neurogenesis*
Transcription Factors / metabolism
|
| IF |
14.415
|
| 引用数 |
1
|
| リソース情報 |
| ショウジョウバエ |
DGRC#109023 |
