| RRC ID |
59219
|
| 著者 |
Meng Q, Watanabe Y, Suzuki R, Oguri R, Tatsukawa H, Hitomi K.
|
| タイトル |
Transglutaminase orthologues in medaka fish - biochemical characterization and establishment of gene-deficient mutants.
|
| ジャーナル |
Anal Biochem
|
| Abstract |
By genome analysis, seven homologous genes (orthologues) of human transglutaminases (TGases) have been identified in medaka fish (Oryzias latipes), some of which clearly corresponded to Factor XIII, TG1, and TG2. The enzymatically active-recombinant proteins for these medaka TGases have been successfully produced in bacteria or baculovirus-infected insect cell systems. Specific antibodies have been prepared and used in immunohistochemical analyses to reveal tissue distribution. Furthermore, gene-deficient medaka mutants for the genes encoding Factor XIII and TG1 have been established together with analysis of their phenotypes. Retarded cross-linking of fibrin and higher sensitivity to osmolality are observed when each gene is knocked-out. In this review, we summarize these biochemical features and the phenotypes of these gene-deficient fish.
|
| 巻・号 |
604
|
| ページ |
113610
|
| 公開日 |
2020-9-1
|
| DOI |
10.1016/j.ab.2020.113610
|
| PII |
S0003-2697(19)31128-5
|
| PMID |
32014415
|
| MeSH |
Animals
Fish Proteins / genetics
Fish Proteins / metabolism*
Gene Knockout Techniques
Oryzias / genetics*
Oryzias / metabolism
Phenotype
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Transglutaminases / genetics
Transglutaminases / metabolism*
|
| IF |
2.877
|
| 引用数 |
0
|
| リソース情報 |
| メダカ |
cDNA
MT830 OK-Cab
MT997 OlTGK2(W368X)
MT1027 OlTGT(del10)
MT1116 FXIIIAdel7
MT1117 FXIIIAdel8
MT1118 OlTGK1(ins2)
MT1119 OlTGT(ins44) |
