論文 - 詳細
| RRC ID | 59326 |
|---|---|
| 著者 | Chen YZ, Klöditz K, Lee ES, Nguyen DP, Yuan Q, Johnson J, Lee-Yow Y, Hall A, Mitani S, Xia NS, Fadeel B, Xue D. |
| タイトル | Structure and function analysis of the C. elegans aminophospholipid translocase TAT-1. |
| ジャーナル | J Cell Sci |
| Abstract |
The Caenorhabditis elegans aminophospholipid translocase TAT-1 maintains phosphatidylserine (PS) asymmetry in the plasma membrane and regulates endocytic transport. Despite these important functions, the structure-function relationship of this protein is poorly understood. Taking advantage of the tat-1 mutations identified by the C. elegans million mutation project, we investigated the effects of 16 single amino acid substitutions on the two functions of the TAT-1 protein. Two substitutions that alter a highly conserved PISL motif in the fourth transmembrane domain and a highly conserved DKTGT phosphorylation motif, respectively, disrupt both functions of TAT-1, leading to a vesicular gut defect and ectopic PS exposure on the cell surface, whereas most other substitutions across the TAT-1 protein, often predicted to be deleterious by bioinformatics programs, do not affect the functions of TAT-1. These results provide in vivo evidence for the importance of the PISL and DKTGT motifs in P4-type ATPases and improve our understanding of the structure-function relationship of TAT-1. Our study also provides an example of how the C. elegans million mutation project helps decipher the structure, functions, and mechanisms of action of important genes. |
| 巻・号 | 132(5) |
| 公開日 | 2019-2-28 |
| DOI | 10.1242/jcs.227660 |
| PII | jcs.227660 |
| PMID | 30683797 |
| PMC | PMC6432707 |
| MeSH | Amino Acid Motifs / genetics Animals Animals, Genetically Modified Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Computational Biology Endocytosis Intestines / physiology* Mutation / genetics Phenotype Phospholipid Transfer Proteins / genetics Phospholipid Transfer Proteins / metabolism* Protein Conformation Protein Transport Structure-Activity Relationship |
| IF | 4.517 |
| 引用数 | 0 |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3117 |