論文 - 詳細
| RRC ID | 59425 |
|---|---|
| 著者 | Yang B, Xu X, Russell L, Sullenberger MT, Yanowitz JL, Maine EM. |
| タイトル | A DNA repair protein and histone methyltransferase interact to promote genome stability in the Caenorhabditis elegans germ line. |
| ジャーナル | PLoS Genet |
| Abstract |
Histone modifications regulate gene expression and chromosomal events, yet how histone-modifying enzymes are targeted is poorly understood. Here we report that a conserved DNA repair protein, SMRC-1, associates with MET-2, the C. elegans histone methyltransferase responsible for H3K9me1 and me2 deposition. We used molecular, genetic, and biochemical methods to investigate the biological role of SMRC-1 and to explore its relationship with MET-2. SMRC-1, like its mammalian ortholog SMARCAL1, provides protection from DNA replication stress. SMRC-1 limits accumulation of DNA damage and promotes germline and embryonic viability. MET-2 and SMRC-1 localize to mitotic and meiotic germline nuclei, and SMRC-1 promotes an increase in MET-2 abundance in mitotic germline nuclei upon replication stress. In the absence of SMRC-1, germline H3K9me2 generally decreases after multiple generations at high culture temperature. Genetic data are consistent with MET-2 and SMRC-1 functioning together to limit replication stress in the germ line and in parallel to promote other germline processes. We hypothesize that loss of SMRC-1 activity causes chronic replication stress, in part because of insufficient recruitment of MET-2 to nuclei. |
| 巻・号 | 15(2) |
| ページ | e1007992 |
| 公開日 | 2019-2-1 |
| DOI | 10.1371/journal.pgen.1007992 |
| PII | PGENETICS-D-18-01292 |
| PMID | 30794539 |
| PMC | PMC6402707 |
| MeSH | Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / metabolism* DNA Helicases / genetics DNA Helicases / metabolism* DNA Repair DNA Replication Female Genomic Instability* Germ Cells / metabolism* Histone-Lysine N-Methyltransferase / metabolism* Histones / metabolism Male Protein Binding |
| IF | 5.224 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm5021 tm892 |