RRC ID 59425
著者 Yang B, Xu X, Russell L, Sullenberger MT, Yanowitz JL, Maine EM.
タイトル A DNA repair protein and histone methyltransferase interact to promote genome stability in the Caenorhabditis elegans germ line.
ジャーナル PLoS Genet
Abstract Histone modifications regulate gene expression and chromosomal events, yet how histone-modifying enzymes are targeted is poorly understood. Here we report that a conserved DNA repair protein, SMRC-1, associates with MET-2, the C. elegans histone methyltransferase responsible for H3K9me1 and me2 deposition. We used molecular, genetic, and biochemical methods to investigate the biological role of SMRC-1 and to explore its relationship with MET-2. SMRC-1, like its mammalian ortholog SMARCAL1, provides protection from DNA replication stress. SMRC-1 limits accumulation of DNA damage and promotes germline and embryonic viability. MET-2 and SMRC-1 localize to mitotic and meiotic germline nuclei, and SMRC-1 promotes an increase in MET-2 abundance in mitotic germline nuclei upon replication stress. In the absence of SMRC-1, germline H3K9me2 generally decreases after multiple generations at high culture temperature. Genetic data are consistent with MET-2 and SMRC-1 functioning together to limit replication stress in the germ line and in parallel to promote other germline processes. We hypothesize that loss of SMRC-1 activity causes chronic replication stress, in part because of insufficient recruitment of MET-2 to nuclei.
巻・号 15(2)
ページ e1007992
公開日 2019-2-1
DOI 10.1371/journal.pgen.1007992
PII PGENETICS-D-18-01292
PMID 30794539
PMC PMC6402707
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / metabolism* DNA Helicases / genetics DNA Helicases / metabolism* DNA Repair DNA Replication Female Genomic Instability* Germ Cells / metabolism* Histone-Lysine N-Methyltransferase / metabolism* Histones / metabolism Male Protein Binding
IF 5.224
引用数 5
リソース情報
線虫 tm5021 tm892