論文 - 詳細
| RRC ID | 59481 |
|---|---|
| 著者 | Dodson AE, Kennedy S. |
| タイトル | Germ Granules Coordinate RNA-Based Epigenetic Inheritance Pathways. |
| ジャーナル | Dev Cell |
| Abstract |
Germ granules are biomolecular condensates that promote germ cell totipotency in animals. In C. elegans, MEG-3 and MEG-4 function redundantly to assemble germ granules in germline blastomeres. Here, we show that meg-3/4 mutant animals exhibit defects in RNA interference (RNAi) that are transgenerationally disconnected from the meg-3/4 genotype. Similar non-Mendelian inheritance is associated with other mutations disrupting germ granule formation, indicating that loss of germ granules is the likely cause of the observed disconnects between genotype and phenotype. meg-3/4 animals produce aberrant siRNAs that are propagated for ≅10 generations in wild-type descendants of meg-3/4 ancestors. Aberrant siRNAs inappropriately and heritably silence germline-expressed genes including the RNAi gene sid-1, suggesting that transgenerational silencing of sid-1 underlies inherited defects in RNAi. We conclude that one function of germ granules is to organize RNA-based epigenetic inheritance pathways and that germ granule loss has consequences that persist for many generations. |
| 巻・号 | 50(6) |
| ページ | 704-715.e4 |
| 公開日 | 2019-9-23 |
| DOI | 10.1016/j.devcel.2019.07.025 |
| PII | S1534-5807(19)30631-8 |
| PMID | 31402284 |
| PMC | PMC7316138 |
| MeSH | Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / metabolism Cytoplasmic Granules / metabolism* Epigenesis, Genetic* Gene Expression Regulation Genotype Germ Cells / metabolism* Inheritance Patterns / genetics* Phenotype RNA Interference* RNA, Small Interfering / genetics |
| IF | 9.19 |
| 引用数 | 6 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm4259 |