論文 - 詳細
| RRC ID | 59523 |
|---|---|
| 著者 | Krahn N, Meier M, Reuten R, Koch M, Stetefeld J, Patel TR. |
| タイトル | Solution Structure of C. elegans UNC-6: A Nematode Paralogue of the Axon Guidance Protein Netrin-1. |
| ジャーナル | Biophys J |
| Abstract |
UNCoordinated-6 (UNC-6) was the first member of the netrin family to be discovered in Caenorhabditis elegans. With homology to human netrin-1, it is a key signaling molecule involved in directing axon migration in nematodes. Similar to netrin-1, UNC-6 interacts with multiple receptors (UNC-5 and UNC-40, specifically) to guide axon migration in development. As a result of the distinct evolutionary path of UNC-6 compared to vertebrate netrins, we decided to employ an integrated approach to study its solution behavior and compare it to the high-resolution structure we previously published on vertebrate netrins. Dynamic light scattering and analytical ultracentrifugation on UNC-6 (with and without its C-domain) solubilized in a low-ionic strength buffer suggested that UNC-6 forms high-order oligomers. An increase in the buffer ionic strength resulted in a more homogeneous preparation of UNC-6, that was used for subsequent solution x-ray scattering experiments. Our biophysical analysis of UNC-6 ΔC solubilized in a high-ionic strength buffer suggested that it maintains a similar head-to-stalk arrangement as netrins -1 and -4. This phenomenon is thought to play a role in the signaling behavior of UNC-6 and its ability to move throughout the extracellular matrix. |
| 巻・号 | 116(11) |
| ページ | 2121-2130 |
| 公開日 | 2019-6-4 |
| DOI | 10.1016/j.bpj.2019.04.033 |
| PII | S0006-3495(19)30377-7 |
| PMID | 31103237 |
| PMC | PMC6554493 |
| MeSH | Amino Acid Sequence Axon Guidance* Caenorhabditis elegans Proteins / chemistry* Caenorhabditis elegans Proteins / metabolism Evolution, Molecular Netrin-1 / chemistry* Netrin-1 / metabolism Netrins / chemistry* Netrins / metabolism Osmolar Concentration Protein Domains Sequence Homology, Amino Acid* Solutions |
| IF | 3.665 |
| 引用数 | 1 |
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