論文 - 詳細
| RRC ID | 59527 |
|---|---|
| 著者 | Lara-Gonzalez P, Moyle MW, Budrewicz J, Mendoza-Lopez J, Oegema K, Desai A. |
| タイトル | The G2-to-M Transition Is Ensured by a Dual Mechanism that Protects Cyclin B from Degradation by Cdc20-Activated APC/C. |
| ジャーナル | Dev Cell |
| Abstract |
In the eukaryotic cell cycle, a threshold level of cyclin B accumulation triggers the G2-to-M transition, and subsequent cyclin B destruction triggers mitotic exit. The anaphase-promoting complex/cyclosome (APC/C) is the E3 ubiquitin ligase that, together with its co-activator Cdc20, targets cyclin B for destruction during mitotic exit. Here, we show that two pathways act in concert to protect cyclin B from Cdc20-activated APC/C in G2, in order to enable cyclin B accumulation and the G2-to-M transition. The first pathway involves the Mad1-Mad2 spindle checkpoint complex, acting in a distinct manner from checkpoint signaling after mitotic entry but employing a common molecular mechanism-the promotion of Mad2-Cdc20 complex formation. The second pathway involves cyclin-dependent kinase phosphorylation of Cdc20, which is known to reduce Cdc20's affinity for the APC/C. Cooperation of these two mechanisms, which target distinct APC/C binding interfaces of Cdc20, enables cyclin B accumulation and the G2-to-M transition. |
| 巻・号 | 51(3) |
| ページ | 313-325.e10 |
| 公開日 | 2019-11-4 |
| DOI | 10.1016/j.devcel.2019.09.005 |
| PII | S1534-5807(19)30734-8 |
| PMID | 31588029 |
| PMC | PMC7778526 |
| MeSH | Anaphase-Promoting Complex-Cyclosome / metabolism* Animals Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism Cdc20 Proteins / metabolism* Cell Line, Tumor Cyclin B / metabolism* Cyclin-Dependent Kinases / metabolism Fertility G2 Phase* Humans Mitosis* Models, Biological Phosphorylation Protein Binding Proteolysis* Spindle Apparatus / metabolism |
| IF | 9.19 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 14 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1969 tm2027 |