RRC ID 59553
著者 Medwig-Kinney TN, Smith JJ, Palmisano NJ, Tank S, Zhang W, Matus DQ.
タイトル A developmental gene regulatory network for C. elegans anchor cell invasion.
ジャーナル Development
Abstract Cellular invasion is a key part of development, immunity and disease. Using an in vivo model of Caenorhabditis elegans anchor cell invasion, we characterize the gene regulatory network that promotes cell invasion. The anchor cell is initially specified in a stochastic cell fate decision mediated by Notch signaling. Previous research has identified four conserved transcription factors, fos-1 (Fos), egl-43 (EVI1/MEL), hlh-2 (E/Daughterless) and nhr-67 (NR2E1/TLX), that mediate anchor cell specification and/or invasive behavior. Connections between these transcription factors and the underlying cell biology that they regulate are poorly understood. Here, using genome editing and RNA interference, we examine transcription factor interactions before and after anchor cell specification. Initially, these transcription factors function independently of one another to regulate LIN-12 (Notch) activity. Following anchor cell specification, egl-43, hlh-2 and nhr-67 function largely parallel to fos-1 in a type I coherent feed-forward loop with positive feedback to promote invasion. Together, these results demonstrate that the same transcription factors can function in cell fate specification and differentiated cell behavior, and that a gene regulatory network can be rapidly assembled to reinforce a post-mitotic, pro-invasive state.
巻・号 147(1)
公開日 2020-1-2
DOI 10.1242/dev.185850
PII dev.185850
PMID 31806663
PMC PMC6983719
MeSH Animals Basic Helix-Loop-Helix Transcription Factors / metabolism Caenorhabditis elegans / cytology Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / metabolism Cell Cycle Cell Lineage* / genetics Cell Movement / genetics* Female Gene Expression Regulation, Developmental* Gene Regulatory Networks* Green Fluorescent Proteins Protein Binding Protein Isoforms RNA Interference Receptors, Cytoplasmic and Nuclear / metabolism Receptors, Notch / metabolism Signal Transduction Transcription Factors / metabolism Uterus / cytology Uterus / embryology
IF 5.763
引用数 1
リソース情報
線虫 tm1802