Reference - Detail
| RRC ID | 59555 |
|---|---|
| Author | Min H, Kim JS, Ahn J, Shim YH. |
| Title | Gliadin Intake Causes Disruption of the Intestinal Barrier and an Increase in Germ Cell Apoptosis in A Caenorhabditis Elegans Model. |
| Journal | Nutrients |
| Abstract |
Gliadin is a major protein component of gluten and causes gluten toxicity through intestinal stress. We previously showed that gliadin intake induces oxidative stress in the intestine and reduces fertility in a Caenorhabditis elegans model. To elucidate the possible link between intestinal stress and reproduction, changes in the intestine and germ cells of C. elegans after gliadin intake were examined at the molecular level. Gliadin intake increased reactive oxygen species (ROS) production in the intestine, decreased intestinal F-actin levels, and increased germ cell apoptosis. These gliadin-triggered effects were suppressed by antioxidant treatment. These results suggest that ROS production in the intestine induced by gliadin intake causes disruption of intestinal integrity and increases germ cell apoptosis. Gliadin-induced germ cell apoptosis (GIGA) was suppressed by depletion of cep-1, ced-13, egl-1, or mpk-1. However, HUS-1 was not activated, suggesting that GIGA is activated through the mitogen-activated protein kinase (MAPK) pathway and is CEP-1-dependent but is a separate pathway from that controlling the DNA damage response. Taken together, our results suggest that gliadin causes intestinal barrier disruption through ROS production and interacts with the germ cells to reduce fertility through GIGA. |
| Volume | 11(11) |
| Published | 2019-10-27 |
| DOI | 10.3390/nu11112587 |
| PII | nu11112587 |
| PMID | 31717869 |
| PMC | PMC6893585 |
| MeSH | Animals Apoptosis / drug effects* Caenorhabditis elegans Cell Survival / drug effects Cells, Cultured Germ Cells / drug effects Gliadin / chemistry Gliadin / toxicity* Intestinal Mucosa / drug effects* Intestinal Mucosa / metabolism Mice RAW 264.7 Cells Reactive Oxygen Species / metabolism |
| IF | 4.171 |
| Times Cited | 1 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm536 |