| RRC ID |
596
|
| 著者 |
Dieterle M, Thomann A, Renou JP, Parmentier Y, Cognat V, Lemonnier G, Müller R, Shen WH, Kretsch T, Genschik P.
|
| タイトル |
Molecular and functional characterization of Arabidopsis Cullin 3A.
|
| ジャーナル |
Plant J
|
| Abstract |
Cullin proteins, which belong to multigenic families in all eukaryotes, associate with other proteins to form ubiquitin protein ligases (E3s) that target substrates for proteolysis by the 26S proteasome. Here, we present the molecular and genetic characterization of a plant Cullin3. In contrast to fungi and animals, the genome of the model plant Arabidopsis thaliana contains two related CUL3 genes, called CUL3A and CUL3B. We found that CUL3A is ubiquitously expressed in plants and is able to interact with the ring-finger protein RBX1. A genomic search revealed the existence of at least 76 BTB-domain proteins in Arabidopsis belonging to 11 major families. Yeast two-hybrid experiments indicate that representative members of certain families are able to physically interact with both CUL3A and CUL3B, suggesting that Arabidopsis CUL3 forms E3 protein complexes with certain BTB domain proteins. In order to determine the function of CUL3A, we used a reverse genetic approach. The cul3a null mutant flowers slightly later than the control plants. Furthermore, this mutant exhibits a reduced sensitivity of the inhibition of hypocotyl growth in far-red light and miss-expresses COP1. The viability of the mutant plants suggests functional redundancy between the two CUL3 genes in Arabidopsis.
|
| 巻・号 |
41(3)
|
| ページ |
386-99
|
| 公開日 |
2005-2-1
|
| DOI |
10.1111/j.1365-313X.2004.02302.x
|
| PII |
TPJ2302
|
| PMID |
15659098
|
| MeSH |
Arabidopsis / genetics*
Arabidopsis / physiology
Arabidopsis Proteins
Carrier Proteins / genetics*
Carrier Proteins / physiology
Cullin Proteins
Flowers
Gene Expression Regulation, Plant
Genes, Homeobox
Hypocotyl / genetics
Hypocotyl / physiology
Light
Multigene Family
Mutation
Phenotype
Phylogeny
Protein Structure, Tertiary
Saccharomyces cerevisiae
Two-Hybrid System Techniques
|
| IF |
6.141
|
| 引用数 |
63
|
|
WOS 分野
|
PLANT SCIENCES
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pda09325
pda01157
pda09658
pda09496
pda04359
pda08737 |
