RRC ID 59600
著者 Steuer Costa W, Van der Auwera P, Glock C, Liewald JF, Bach M, Schüler C, Wabnig S, Oranth A, Masurat F, Bringmann H, Schoofs L, Stelzer EHK, Fischer SC, Gottschalk A.
タイトル A GABAergic and peptidergic sleep neuron as a locomotion stop neuron with compartmentalized Ca2+ dynamics.
ジャーナル Nat Commun
Abstract Animals must slow or halt locomotion to integrate sensory inputs or to change direction. In Caenorhabditis elegans, the GABAergic and peptidergic neuron RIS mediates developmentally timed quiescence. Here, we show RIS functions additionally as a locomotion stop neuron. RIS optogenetic stimulation caused acute and persistent inhibition of locomotion and pharyngeal pumping, phenotypes requiring FLP-11 neuropeptides and GABA. RIS photoactivation allows the animal to maintain its body posture by sustaining muscle tone, yet inactivating motor neuron oscillatory activity. During locomotion, RIS axonal Ca2+ signals revealed functional compartmentalization: Activity in the nerve ring process correlated with locomotion stop, while activity in a branch correlated with induced reversals. GABA was required to induce, and FLP-11 neuropeptides were required to sustain locomotion stop. RIS attenuates neuronal activity and inhibits movement, possibly enabling sensory integration and decision making, and exemplifies dual use of one cell across development in a compact nervous system.
巻・号 10(1)
ページ 4095
公開日 2019-9-10
DOI 10.1038/s41467-019-12098-5
PII 10.1038/s41467-019-12098-5
PMID 31506439
PMC PMC6736843
MeSH Animals Axons / metabolism Caenorhabditis elegans / cytology Caenorhabditis elegans / physiology* Calcium / metabolism* Cholinergic Neurons / physiology GABAergic Neurons / metabolism* Gap Junctions / metabolism Light Locomotion / physiology* Models, Biological Motor Neurons / physiology Muscles / cytology Neuropeptides / metabolism* Phenotype Signal Transduction Sleep / physiology* gamma-Aminobutyric Acid / metabolism
IF 11.878
引用数 3
リソース情報
線虫 tm2706