論文 - 詳細
| RRC ID | 59637 |
|---|---|
| 著者 | Zhao Y, Wang H, Poole RJ, Gems D. |
| タイトル | A fln-2 mutation affects lethal pathology and lifespan in C. elegans. |
| ジャーナル | Nat Commun |
| Abstract |
Differences in genetic background in model organisms can have complex effects on phenotypes of interest. We previously reported a difference in hermaphrodite lifespan between two wild-type lines widely used by C. elegans researchers (N2 hermaphrodite and male stocks). Here, using pathology-based approaches and genome sequencing, we identify the cause of this difference as a nonsense mutation in the filamin gene fln-2 in the male stock, which reduces early mortality caused by pharyngeal infection. We show how fln-2 variation explains previous discrepancies involving effects of sir-2.1 (sirtuin deacetylase) on ageing, and show that in a fln-2(+) background, sir-2.1 over-expression causes an FUDR (DNA synthesis inhibitor)-dependent reduction in pharyngeal infection and increase in lifespan. In addition we show how fln-2 variation confounds effects on lifespan of daf-2 (insulin/IGF-1 signalling), daf-12 (steroid hormone signalling), and eat-2 (putative dietary restriction). These findings underscore the importance of identifying and controlling genetic background variation. |
| 巻・号 | 10(1) |
| ページ | 5087 |
| 公開日 | 2019-11-8 |
| DOI | 10.1038/s41467-019-13062-z |
| PII | 10.1038/s41467-019-13062-z |
| PMID | 31704915 |
| PMC | PMC6841690 |
| MeSH | Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics Codon, Nonsense Epistasis, Genetic / genetics* Filamins / genetics* Genetic Background Hermaphroditic Organisms Longevity / genetics* Male Models, Animal Pharyngitis / genetics* Pharyngitis / mortality Receptor, Insulin / genetics Receptors, Cytoplasmic and Nuclear / genetics Receptors, Nicotinic / genetics Sirtuins |
| IF | 11.878 |
| 引用数 | 3 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm4687 |