論文 - 詳細
| RRC ID | 59652 |
|---|---|
| 著者 | Minato KI, Laan LC, van Die I, Mizuno M. |
| タイトル | Pleurotus citrinopileatus polysaccharide stimulates anti-inflammatory properties during monocyte-to-macrophage differentiation. |
| ジャーナル | Int J Biol Macromol |
| Abstract |
Polysaccharides from edible mushrooms possess important immunomodulating effects on immune cells including monocytes and macrophages. Macrophages activated by LPS/IFNγ are polarized toward inflammatory macrophages, whereas the anti-inflammatory properties of alternative activated macrophages play an important regulatory role in the innate immune system. We here show that the Pleurotus citrinopileatus mushroom polysaccharide (PCPS) can modulate the monocyte-to-macrophage differentiation early at the monocyte stage. Using both human THP-1 monocytic cells as well as human peripheral monocytes, we showed that PCPS inhibits the secreted levels of the pro-inflammatory cytokines TNF and IL-6, after stimulation of macrophages derived from PCPS-treated monocytes, with IFNγ + LPS. In addition, the glucan induced a tendency to increase the secreted levels of the anti-inflammatory cytokine IL-10, enhanced the expression levels of CCL2 and CCL8 mRNAs, and inhibited expression of CCR2 mRNA in the IFNγ/LPS activated macrophages. Interestingly, these data suggest that PCPS can induce a long-lasting anti-inflammatory effect in monocytes. Treatment of monocytes with laminarin and antibodies against Dectin-1 and TLR2 during PCPS treatment affected the glucan-modulated macrophage differentiation. In summary, the results of this study indicate that the glucan directs the differentiation of monocytes toward a macrophage cell population with reduced pro-inflammatory capacity via Dectin-1 and TLR2. |
| 巻・号 | 122 |
| ページ | 705-712 |
| 公開日 | 2019-2-1 |
| DOI | 10.1016/j.ijbiomac.2018.10.157 |
| PII | S0141-8130(18)33535-9 |
| PMID | 30392853 |
| MeSH | Anti-Inflammatory Agents / pharmacology* Cell Differentiation / drug effects* Cell Line Chemokines / biosynthesis Fungal Polysaccharides / pharmacology* Humans Interferon-gamma / pharmacology Lectins, C-Type / metabolism Lipopolysaccharides / pharmacology Macrophages / cytology* Macrophages / drug effects Macrophages / immunology Macrophages / metabolism Monocytes / cytology* Monocytes / drug effects Phenotype Pleurotus / chemistry* Signal Transduction / drug effects |
| IF | 4.784 |
| 引用数 | 8 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | THP-1(RCB1189) |