| RRC ID |
59670
|
| 著者 |
Kim HJ, Kim HJ, Kim MK, Bae MK, Sung HY, Ahn JH, Kim YH, Kim SC, Ju W.
|
| タイトル |
SPSB1 enhances ovarian cancer cell survival by destabilizing p21.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
SPRY domain-containing SOCS box protein 1 (SPSB1) is an E3 ligase adaptor protein with unknown functions in cancer cells. In this study, we found that SPSB1 knockdown markedly decreased the viability and migration of ovarian cancer cells, while ectopic SPSB1 overexpression in IL-3-dependent Ba/F3 cells significantly increased their proliferation rate compared with empty vector-transfected cells. SPSB1 knockdown significantly elevated p21 protein and mRNA levels and induced apoptosis in ovarian cancer cells, as evidenced by increased levels of cleaved PARP and decreased levels of Bcl-2. Notably, mechanistic investigations revealed that SPSB1 accelerated p21 destabilization by directly interacting with p21 and promoting its ubiquitin-mediated proteasomal degradation. Taken together, our findings provide novel insights into the role of SPSB1 in ovarian cancer cells.
|
| 巻・号 |
510(3)
|
| ページ |
364-369
|
| 公開日 |
2019-3-12
|
| DOI |
10.1016/j.bbrc.2019.01.088
|
| PII |
S0006-291X(19)30116-0
|
| PMID |
30712944
|
| MeSH |
Animals
Apoptosis
Cell Cycle Checkpoints
Cell Line, Tumor
Cell Movement
Cell Proliferation
Cell Survival
Cyclin-Dependent Kinase Inhibitor p21 / metabolism*
Female
Gene Silencing
Hematopoietic Stem Cells / cytology
Hematopoietic Stem Cells / metabolism
Humans
Mice
Ovarian Neoplasms / metabolism*
Ovarian Neoplasms / pathology
Proteasome Endopeptidase Complex / metabolism
Protein Stability
Suppressor of Cytokine Signaling Proteins / genetics
Suppressor of Cytokine Signaling Proteins / metabolism
Suppressor of Cytokine Signaling Proteins / physiology*
Ubiquitin / metabolism
|
| IF |
2.705
|
| 引用数 |
2
|
| リソース情報 |
| ヒト・動物細胞 |
Ba/F3(RCB0805) |
