Reference - Detail
| RRC ID | 59692 |
|---|---|
| Author | Tong L, Matsuura E, Takahashi M, Nagano T, Kawazu K. |
| Title | Effects of Anti-Glaucoma Prostaglandin Ophthalmic Solutions on Cultured Human Corneal Epithelial Cells. |
| Journal | Curr Eye Res |
| Abstract |
Purpose: We compare the cytotoxicity of anti-glaucoma prostaglandin ophthalmic solutions on human corneal epithelial cells and elucidate mechanisms of toxicity. Methods: Cell viability was examined using MTS assay, and morphological changes of the cells were observed. Induction of necrosis/apoptosis was measured by colorimetric caspase assay. The production of Reactive oxygen species (ROS) and release of cytokines were analyzed using 2', 7'-dichlorodihydrofluorescein diacetate and bead-based indirect immunofluorescent assay, respectively. Results: Xalatan, Lumigan 0.01%, and Lumigan 0.03% decreased cell viability and induced morphological changes. Xalatan and Lumigan 0.01% induced necrosis. Xalatan, Lumigan 0.01%, Lumigan 0.03%, and Taflotan stimulated ROS production. Travatan and Lumigan 0.03% increased concentrations of Interleukin (IL)-6 and IL-8 in culture media. Conclusions: Xalatan and Lumigan 0.01% ophthalmic solutions demonstrated potent cytotoxicity compared with Lumigan 0.03%, Travatan, Taflotan, and Taflotan UD. Taflotan UD, compared to Taflotan 0.0015%, induced less oxidative stress and apoptotic signalling. The cytotoxicity might be partly associated with benzalkonium chloride. |
| Volume | 44(8) |
| Pages | 856-862 |
| Published | 2019-8-1 |
| DOI | 10.1080/02713683.2019.1597127 |
| PMID | 30884982 |
| MeSH | Antihypertensive Agents / pharmacology* Bimatoprost / pharmacology Cell Survival Cells, Cultured Cytokines / metabolism Epithelium, Corneal / drug effects* Epithelium, Corneal / metabolism Epithelium, Corneal / pathology Fluorescent Antibody Technique, Indirect Glaucoma / drug therapy Humans Latanoprost / pharmacology* Ophthalmic Solutions Prostaglandins F / pharmacology* Reactive Oxygen Species / metabolism Travoprost / pharmacology* |
| IF | 1.672 |
| Times Cited | 2 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 10 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HCE-T(RCB2280) |