Reference - Detail
| RRC ID | 59707 |
|---|---|
| Author | Yokoyama T, Machida K, Iwasaki W, Shigeta T, Nishimoto M, Takahashi M, Sakamoto A, Yonemochi M, Harada Y, Shigematsu H, Shirouzu M, Tadakuma H, Imataka H, Ito T. |
| Title | HCV IRES Captures an Actively Translating 80S Ribosome. |
| Journal | Mol Cell |
| Abstract |
Translation initiation of hepatitis C virus (HCV) genomic RNA is induced by an internal ribosome entry site (IRES). Our cryoelectron microscopy (cryo-EM) analysis revealed that the HCV IRES binds to the solvent side of the 40S platform of the cap-dependently translating 80S ribosome. Furthermore, we obtained the cryo-EM structures of the HCV IRES capturing the 40S subunit of the IRES-dependently translating 80S ribosome. In the elucidated structures, the HCV IRES "body," consisting of domain III except for subdomain IIIb, binds to the 40S subunit, while the "long arm," consisting of domain II, remains flexible and does not impede the ongoing translation. Biochemical experiments revealed that the cap-dependently translating ribosome becomes a better substrate for the HCV IRES than the free ribosome. Therefore, the HCV IRES is likely to efficiently induce the translation initiation of its downstream mRNA with the captured translating ribosome as soon as the ongoing translation terminates. |
| Volume | 74(6) |
| Pages | 1205-1214.e8 |
| Published | 2019-6-20 |
| DOI | 10.1016/j.molcel.2019.04.022 |
| PII | S1097-2765(19)30314-4 |
| PMID | 31080011 |
| MeSH | Binding Sites Cryoelectron Microscopy Eukaryotic Initiation Factors / chemistry* Eukaryotic Initiation Factors / genetics Eukaryotic Initiation Factors / metabolism HEK293 Cells Hepacivirus / genetics* Hepacivirus / metabolism Host-Pathogen Interactions Humans Internal Ribosome Entry Sites Models, Molecular Nucleic Acid Conformation Peptide Chain Initiation, Translational* RNA, Viral / chemistry* RNA, Viral / genetics RNA, Viral / metabolism Ribosome Subunits, Large, Eukaryotic / genetics Ribosome Subunits, Large, Eukaryotic / metabolism Ribosome Subunits, Large, Eukaryotic / ultrastructure* Ribosome Subunits, Small, Eukaryotic / genetics Ribosome Subunits, Small, Eukaryotic / metabolism Ribosome Subunits, Small, Eukaryotic / ultrastructure* |
| IF | 14.548 |
| Times Cited | 5 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 37 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | 293T(RCB2202) |