RRC ID 59723
Author Park E, Kim J, Kim MC, Yeo S, Kim J, Park S, Jo M, Choi CW, Jin HS, Lee SW, Li WY, Lee JW, Park JH, Huh D, Jeong SY.
Title Anti-Osteoporotic Effects of Kukoamine B Isolated from Lycii Radicis Cortex Extract on Osteoblast and Osteoclast Cells and Ovariectomized Osteoporosis Model Mice.
Journal Int J Mol Sci
Abstract Osteoporosis is an abnormal bone remodeling condition characterized by decreased bone density, which leads to high risks of fracture. Previous study has demonstrated that Lycii Radicis Cortex (LRC) extract inhibits bone loss in ovariectomized (OVX) mice by enhancing osteoblast differentiation. A bioactive compound, kukoamine B (KB), was identified from fractionation of an LRC extract as a candidate component responsible for an anti-osteoporotic effect. This study investigated the anti-osteoporotic effects of KB using in vitro and in vivo osteoporosis models. KB treatment significantly increased the osteoblastic differentiation and mineralized nodule formation of osteoblastic MC3T3-E1 cells, while it significantly decreased the osteoclast differentiation of primary-cultured monocytes derived from mouse bone marrow. The effects of KB on osteoblastic and osteoclastic differentiations under more physiological conditions were also examined. In the co-culture of MC3T3-E1 cells and monocytes, KB promoted osteoblast differentiation but did not affect osteoclast differentiation. In vivo experiments revealed that KB significantly inhibited OVX-induced bone mineral density loss and restored the impaired bone structural properties in osteoporosis model mice. These results suggest that KB may be a potential therapeutic candidate for the treatment of osteoporosis.
Volume 20(11)
Published 2019-6-6
DOI 10.3390/ijms20112784
PII ijms20112784
PMID 31174394
PMC PMC6600412
MeSH Animals Bone Density Conservation Agents / pharmacology Bone Density Conservation Agents / therapeutic use* Bone Marrow Cells / cytology Bone Marrow Cells / drug effects Caffeic Acids / pharmacology Caffeic Acids / therapeutic use* Cell Differentiation Cell Line Cells, Cultured Drugs, Chinese Herbal / chemistry Female Mice Osteoblasts / cytology Osteoblasts / drug effects* Osteoclasts / cytology Osteoclasts / drug effects* Osteoporosis / drug therapy* Osteoporosis / etiology Ovariectomy / adverse effects Spermine / analogs & derivatives* Spermine / pharmacology Spermine / therapeutic use
IF 4.183
Times Cited 1
Human and Animal Cells MC3T3-E1(RCB1126)