論文 - 詳細
RRC ID | 59740 |
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著者 | Iriguchi S, Kaneko S. |
タイトル | In Vitro Differentiation of T Cells: From Human Embryonic Stem Cells and Induced Pluripotent Stem Cells. |
ジャーナル | Methods Mol Biol |
Abstract |
In this chapter, we describe a protocol for hematopoietic differentiation of human pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) derived from non-T cells, followed by the differentiation of the T-cell lineage. Derivation of T cells from PSCs involves three steps: induction of PSCs to hematopoietic progenitor cells (HPCs), differentiation of HPCs into progenitor T cells, and maturation of progenitor T cells into mature T cells (CD8 single-positive (SP) or CD4 SP). |
巻・号 | 2048 |
ページ | 59-70 |
公開日 | 2019-1-1 |
DOI | 10.1007/978-1-4939-9728-2_6 |
PMID | 31396929 |
MeSH | Animals Cell Culture Techniques / instrumentation Cell Culture Techniques / methods* Cell Differentiation* Cell Line Cell Separation / instrumentation Cell Separation / methods Coculture Techniques / instrumentation Coculture Techniques / methods Culture Media / metabolism* Fibroblasts Flow Cytometry / instrumentation Flow Cytometry / methods Fluorescent Antibody Technique, Direct / instrumentation Fluorescent Antibody Technique, Direct / methods Hematopoietic Stem Cells / physiology Human Embryonic Stem Cells / physiology Humans Induced Pluripotent Stem Cells / physiology Intercellular Signaling Peptides and Proteins / metabolism Mesenchymal Stem Cells Mice Recombinant Proteins / metabolism T-Lymphocytes / physiology |
引用数 | 0 |
リソース情報 | |
ヒト・動物細胞 | 10T1/2(RCB0247) |