RRC ID 59743
著者 Nakayama T, Kobayashi T, Shimpei O, Fukuhara H, Namikawa T, Inoue K, Hanazaki K, Takahashi K, Nakajima M, Tanaka T, Ogura SI.
タイトル Photoirradiation after aminolevulinic acid treatment suppresses cancer cell proliferation through the HO-1/p21 pathway.
ジャーナル Photodiagnosis Photodyn Ther
Abstract BACKGROUND:Photodynamic therapy (PDT) and diagnosis (PDD) using 5-aminolevulinic acid (ALA) to control the production of an intracellular photosensitizer, protoporphyrin IX (PpIX), are in common clinical use. Although various studies have been published regarding cell death analysis after photoirradiation by ALA-PDT, the changes in gene expressions induced by it are yet unclear. Here, we focused on studying gene expression and cell proliferation changes in cancer cells that survive photoirradiation.
METHODS:HEK293 human embryonic kidney cells, MKN45 human gastric cells, and PC-3 human prostate cancer cells were selected for this research. Cell viability was measured using trypan blue and MTT assays. ALA-PDT experiments were performed using a calibrated LED irradiation module. Furthermore, mRNA and protein gene expression analysis were performed using our previously reported methods.
RESULTS:mRNAs of PAI-1, HO-1, and p21 were upregulated after photoirradiation of HEK293, which was suppressed by N-acetyl-L-cysteine, a reactive oxygen species (ROS) scavenger. Primer array results in PC-3 cells and p21 and Ki-67 expression results in both PC-3 and MKN45 cells suggested that photoirradiation suppressed cell proliferation. Cell numbers post-photoirradiation revealed that the proliferation of surviving cells was suppressed in PC-3 and MKN45 cells.
CONCLUSION:ALA-PDD or ALA-PDT can result in rapid ROS-induced cell death and may decrease long-term recurrence rates through several pathways including the HO-1/p21 pathway.
巻・号 28
ページ 10-17
公開日 2019-12-1
DOI 10.1016/j.pdpdt.2019.07.021
PII S1572-1000(19)30284-4
PMID 31404677
MeSH Aminolevulinic Acid / pharmacology* Cell Line, Tumor Cell Proliferation / drug effects Cell Survival / drug effects Cyclin-Dependent Kinase Inhibitor p21 / metabolism Gene Expression HEK293 Cells / drug effects* Heme Oxygenase-1 / metabolism Humans PC-3 Cells / drug effects* Photochemotherapy / methods* Photosensitizing Agents / pharmacology* RNA, Messenger / metabolism Reactive Oxygen Species / metabolism Stomach Neoplasms / drug therapy* Up-Regulation / drug effects
IF 2.589
引用数 1
リソース情報
ヒト・動物細胞 MKN45(RCB1001)