| RRC ID |
59778
|
| Author |
Lee KW, Lee NK, Kim JH, Kang MS, Yoo HY, Kim HH, Um SH, Kim SH.
|
| Title |
Twist1 causes the transcriptional repression of claudin-4 with prognostic significance in esophageal cancer.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Twist1 is a transcription factor that is involved in epithelial-mesenchymal transition by suppressing intercellular adhesion. In this study, we aimed to determine the role of Twist1 in the regulation of claudin-4 expression and investigate its specific mechanisms and clinical implications using human esophageal carcinoma cell lines and tissues. As a result, up-regulation of Twist1 decreased both gene and protein expression levels of endogenous claudin-4 and the suppression was mediated by direct binding of Twist1 to the canonical E-box in the promoter region of claudin-4. In addition, there was a significant inverse correlation of claudin-4 with Twist1 in esophageal cancer tissues. High Twist1 and low claudin-4 expression was associated with the poorest prognosis and was more highly correlated with adverse outcome than any other subgroup with statistical significance (p=0.001). Our results indicate that Twist1 induces the repression of claudin-4 expression during the epithelial-mesenchymal transition in esophageal carcinoma.
|
| Volume |
423(3)
|
| Pages |
454-60
|
| Published |
2012-7-6
|
| DOI |
10.1016/j.bbrc.2012.05.140
|
| PII |
S0006-291X(12)01039-X
|
| PMID |
22668877
|
| MeSH |
Adolescent
Adult
Aged
Base Sequence
Carcinoma, Squamous Cell / genetics
Carcinoma, Squamous Cell / mortality
Carcinoma, Squamous Cell / pathology*
Cell Line, Tumor
Child
Child, Preschool
Claudin-4
Claudins / antagonists & inhibitors
Claudins / genetics*
Disease-Free Survival
Down-Regulation
E-Box Elements
Epithelial-Mesenchymal Transition / genetics*
Esophageal Neoplasms / genetics
Esophageal Neoplasms / mortality
Esophageal Neoplasms / pathology*
Gene Expression Regulation, Neoplastic*
Humans
Infant
Infant, Newborn
Middle Aged
Molecular Sequence Data
Nuclear Proteins / genetics
Nuclear Proteins / metabolism*
Prognosis
Promoter Regions, Genetic
Twist-Related Protein 1 / genetics
Twist-Related Protein 1 / metabolism*
Young Adult
|
| IF |
2.985
|
| Resource |
| Human and Animal Cells |
TE-8(RCB2098)
TE-8(RCB2099) |
