| RRC ID |
598
|
| 著者 |
Gingerich DJ, Gagne JM, Salter DW, Hellmann H, Estelle M, Ma L, Vierstra RD.
|
| タイトル |
Cullins 3a and 3b assemble with members of the broad complex/tramtrack/bric-a-brac (BTB) protein family to form essential ubiquitin-protein ligases (E3s) in Arabidopsis.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
Selective modification of proteins by ubiquitination is directed by diverse families of ubiquitin-protein ligases (or E3s). A large collection of E3s use Cullins (CULs) as scaffolds to form multisubunit E3 complexes in which the CUL binds a target recognition subcomplex and the RBX1 docking protein, which delivers the activated ubiquitin moiety. Arabidopsis and rice contain a large collection of CUL isoforms, indicating that multiple CUL-based E3s exist in plants. Here we show that Arabidopsis CUL3a and CUL3b associate with RBX1 and members of the broad complex/tramtrack/bric-a-brac (BTB) protein family to form BTB E3s. Eighty genes encoding BTB domain-containing proteins were identified in the Arabidopsis genome, indicating that a diverse array of BTB E3s is possible. In addition to the BTB domain, the encoded proteins also contain various other interaction motifs that likely serve as target recognition elements. DNA microarray analyses show that BTB genes are expressed widely in the plant and that tissue-specific and isoform-specific patterns exist. Arabidopsis defective in both CUL3a and CUL3b are embryo-lethal, indicating that BTB E3s are essential for plant development.
|
| 巻・号 |
280(19)
|
| ページ |
18810-21
|
| 公開日 |
2005-5-13
|
| DOI |
10.1074/jbc.M413247200
|
| PII |
S0021-9258(20)67526-3
|
| PMID |
15749712
|
| MeSH |
Amino Acid Motifs
Amino Acid Sequence
Animals
Arabidopsis / metabolism*
Arabidopsis Proteins
Carrier Proteins / chemistry*
Codon
Cullin Proteins
DNA / chemistry
Gene Expression Regulation, Plant
Heterozygote
Homozygote
Humans
Models, Biological
Molecular Sequence Data
Multigene Family
Mutation
Oligonucleotide Array Sequence Analysis
Phenotype
Phylogeny
Protein Binding
Protein Isoforms
Protein Structure, Tertiary
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Ubiquitin / chemistry
Ubiquitin-Protein Ligases / chemistry*
|
| IF |
4.238
|
| 引用数 |
101
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pda00987 |
