| RRC ID |
5990
|
| 著者 |
Cao L, Shitara H, Sugimoto M, Hayashi J, Abe K, Yonekawa H.
|
| タイトル |
New evidence confirms that the mitochondrial bottleneck is generated without reduction of mitochondrial DNA content in early primordial germ cells of mice.
|
| ジャーナル |
PLoS Genet
|
| Abstract |
In mammals, observations of rapid shifts in mitochondrial DNA (mtDNA) variants between generations have led to the creation of the bottleneck theory for the transmission of mtDNA. The bottleneck could be attributed to a marked decline of mtDNA content in germ cells giving rise to the next generation, to a small effective number of mtDNA segregation units resulting from homoplasmic nucleoids rather than the single mtDNA molecule serving as the units of segregation, or to the selective transmission of a subgroup of the mtDNA population to the progeny. We have previously determined mtDNA copy number in single germ cells and shown that the bottleneck occurs without the reduction in germline mtDNA content. Recently one study suggested that the bottleneck is driven by a remarkable decline of mtDNA copies in early primordial germ cells (PGCs), while another study reported that the mtDNA genetic bottleneck results from replication of a subpopulation of the mtDNA genome during postnatal oocyte maturation and not during embryonic oogenesis, despite a detected a reduction in mtDNA content in early PGCs. To clarify these contradictory results, we examined the mtDNA copy number in PGCs isolated from transgenic mice expressing fluorescent proteins specifically in PGCs as in the aforementioned two other studies. We provide clear evidence to confirm that no remarkable reduction in mtDNA content occurs in PGCs and reinforce that the bottleneck is generated without reduction of mtDNA content in germ cells.
|
| 巻・号 |
5(12)
|
| ページ |
e1000756
|
| 公開日 |
2009-12-1
|
| DOI |
10.1371/journal.pgen.1000756
|
| PMID |
19997484
|
| PMC |
PMC2777314
|
| MeSH |
Alkaline Phosphatase / metabolism
Animals
Biomarkers / metabolism
Cell Separation
Chromosomal Proteins, Non-Histone
DNA, Mitochondrial / metabolism*
Embryo, Mammalian / cytology
Embryo, Mammalian / metabolism
Gene Dosage
Gene Expression Regulation, Developmental
Germ Cells / cytology
Germ Cells / enzymology
Germ Cells / metabolism*
Luminescent Proteins / metabolism
Mice
Mice, Transgenic
Mitochondria / genetics*
Positive Regulatory Domain I-Binding Factor 1
Repressor Proteins / genetics
Repressor Proteins / metabolism
Reproducibility of Results
Staining and Labeling
Transcription Factors / metabolism
|
| IF |
5.175
|
| 引用数 |
73
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| 実験動物マウス |
RBRC00821
RBRC01830 |
