RRC ID 59924
著者 Hirama H, Satoh T, Sugiura S, Shin K, Onuki-Nagasaki R, Kanamori T, Inoue T.
タイトル Glass-based organ-on-a-chip device for restricting small molecular absorption.
ジャーナル J Biosci Bioeng
Abstract The use of organ-on-a-chip (OOC) devices is a promising alternative to existing cell-based assays and animal testing in drug discovery. A rapid prototyping method with polydimethylsiloxane (PDMS) is widely used for developing OOC devices. However, because PDMS tends to absorb small hydrophobic molecules, the loss of test compounds in cell-based assays and increases in background fluorescence during observation often lead to biased results in cell-based assays. To address this issue, we have fabricated a glass-based OOC device and characterized the medium flow and molecular absorption properties in comparison with PDMS-based devices. Consequently, we revealed that the glass device generated a stable medium flow, restricted the absorption of small hydrophobic molecules, and showed enhanced cell adhesiveness. This glass device is expected to be applicable to precise cell-based assays to evaluate small hydrophobic molecules, for which PDMS devices cannot be applied because of their absorption of small hydrophobic molecules.
巻・号 127(5)
ページ 641-646
公開日 2019-5-1
DOI 10.1016/j.jbiosc.2018.10.019
PII S1389-1723(18)30641-8
PMID 30473393
MeSH Adsorption Animals Biological Assay / instrumentation* Cell Adhesion Cell Line Dimethylpolysiloxanes / chemistry Glass / chemistry Humans Hydrophobic and Hydrophilic Interactions Lab-On-A-Chip Devices*
IF 2.032
引用数 4
リソース情報
ヒト・動物細胞 Hep G2(RCB1886)