論文 - 詳細
| RRC ID | 59924 |
|---|---|
| 著者 | Hirama H, Satoh T, Sugiura S, Shin K, Onuki-Nagasaki R, Kanamori T, Inoue T. |
| タイトル | Glass-based organ-on-a-chip device for restricting small molecular absorption. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
The use of organ-on-a-chip (OOC) devices is a promising alternative to existing cell-based assays and animal testing in drug discovery. A rapid prototyping method with polydimethylsiloxane (PDMS) is widely used for developing OOC devices. However, because PDMS tends to absorb small hydrophobic molecules, the loss of test compounds in cell-based assays and increases in background fluorescence during observation often lead to biased results in cell-based assays. To address this issue, we have fabricated a glass-based OOC device and characterized the medium flow and molecular absorption properties in comparison with PDMS-based devices. Consequently, we revealed that the glass device generated a stable medium flow, restricted the absorption of small hydrophobic molecules, and showed enhanced cell adhesiveness. This glass device is expected to be applicable to precise cell-based assays to evaluate small hydrophobic molecules, for which PDMS devices cannot be applied because of their absorption of small hydrophobic molecules. |
| 巻・号 | 127(5) |
| ページ | 641-646 |
| 公開日 | 2019-5-1 |
| DOI | 10.1016/j.jbiosc.2018.10.019 |
| PII | S1389-1723(18)30641-8 |
| PMID | 30473393 |
| MeSH | Adsorption Animals Biological Assay / instrumentation* Cell Adhesion Cell Line Dimethylpolysiloxanes / chemistry Glass / chemistry Humans Hydrophobic and Hydrophilic Interactions Lab-On-A-Chip Devices* |
| IF | 2.032 |
| 引用数 | 4 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2(RCB1886) |