RRC ID 59979
Author Hadjivasiliou Z, Moore RE, McIntosh R, Galea GL, Clarke JDW, Alexandre P.
Title Basal Protrusions Mediate Spatiotemporal Patterns of Spinal Neuron Differentiation.
Journal Dev Cell
Abstract During early spinal cord development, neurons of particular subtypes differentiate with a sparse periodic pattern while later neurons differentiate in the intervening space to eventually produce continuous columns of similar neurons. The mechanisms that regulate this spatiotemporal pattern are unknown. In vivo imaging in zebrafish reveals that differentiating spinal neurons transiently extend two long protrusions along the basal surface of the spinal cord before axon initiation. These protrusions express Delta protein, consistent with the hypothesis they influence Notch signaling at a distance of several cell diameters. Experimental reduction of Laminin expression leads to smaller protrusions and shorter distances between differentiating neurons. The experimental data and a theoretical model support the proposal that neuronal differentiation pattern is regulated by transient basal protrusions that deliver temporally controlled lateral inhibition mediated at a distance. This work uncovers a stereotyped protrusive activity of newborn neurons that organize long-distance spatiotemporal patterning of differentiation.
Volume 49(6)
Pages 907-919.e10
Published 2019-6-17
DOI 10.1016/j.devcel.2019.05.035
PII S1534-5807(19)30448-4
PMID 31211994
PMC PMC6584357
MeSH Animals Body Patterning* Cell Communication Cell Differentiation* Embryo, Nonmammalian / cytology* Embryo, Nonmammalian / metabolism Gene Expression Regulation, Developmental Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism Laminin / genetics Laminin / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism Motor Neurons / cytology* Motor Neurons / metabolism Neurogenesis Signal Transduction Spatio-Temporal Analysis Spinal Cord / cytology* Spinal Cord / metabolism Zebrafish / embryology* Zebrafish / metabolism Zebrafish Proteins / genetics Zebrafish Proteins / metabolism
IF 9.19
Times Cited 2
Resource
Zebrafish Tg(vsx1:GFP)