論文 - 詳細
| RRC ID | 59990 |
|---|---|
| 著者 | Paerhati P, Ito A, Yoshioka K, Iwamoto K, Fujiwara S, Horie M, Kawabe Y, Kamihira M. |
| タイトル | Neural differentiation of mouse induced pluripotent stem cells using cadherin gene-engineered PA6 feeder cells. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
Investigating neural differentiation of pluripotent stem cells, including induced pluripotent stem (iPS) cells, is of importance for studying early neural development and providing a potential source of cells for nerve regeneration. Stromal cell-derived inducing activity (SDIA) using PA6 stromal cells promotes neural differentiation of iPS cells. Thus, we hypothesized that cadherin gene-engineered PA6 feeder cells will enhance the performance of SDIA by facilitating cell-cell interactions. Consequently, we created cadherin gene-engineered PA6 cells. Efficiency of neural differentiation from mouse iPS cells on PA6 feeder cells overexpressing E-cadherin gene (46%) or N-cadherin gene (27%) was significantly higher compared with parental PA6 feeder cells (19%). In addition, efficiency of motor neuron differentiation from mouse iPS cells on cadherin-gene engineered feeder cells (E-cadherin, 7.4%; N-cadherin, 11%) was significantly higher compared with parental PA6 feeder cells (4.1%). Altogether, these results indicate that cadherin gene-engineered feeder cells are a potent tool for promoting neural differentiation of pluripotent stem cells. |
| 巻・号 | 127(5) |
| ページ | 633-640 |
| 公開日 | 2019-5-1 |
| DOI | 10.1016/j.jbiosc.2018.10.009 |
| PII | S1389-1723(18)30643-1 |
| PMID | 30391238 |
| MeSH | Animals Cadherins / genetics Cadherins / metabolism Cell Communication Cell Differentiation* Feeder Cells / cytology* Feeder Cells / metabolism Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism Mice Neurogenesis Pluripotent Stem Cells / cytology Stromal Cells / cytology* Stromal Cells / metabolism |
| IF | 2.032 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | iPS-MEF-Ng-20D-17(APS0001) MC3T3-G2/PA6(RCB1127) |