RRC ID 6001
Author Ju SY, Cho KA, Cho SJ, Jung YJ, Woo SY, Seoh JY, Han HS, Ryu KH.
Title Effect of hypoxic treatment on bone marrow cells that are able to migrate to the injured liver.
Journal Cell Biol Int
Abstract Restricted numbers and poor regenerative properties limit the use of adult stem cells. We tested the effect of hypoxic treatment as a method by which to increase cell migration. Bone marrow cells (BMCs) were cultured under oxygen saturations of 0.1, 3, and 20% for 24h. After hypoxic treatment, BMCs of apoptotic fraction were decreased. The expression of CXCR4 was noticeably increased in the hypoxia-treated BMCs and their migration in response to SDF-1alpha was enhanced compared with cells cultured under normoxic condition. Hypoxic BMCs had a higher degree of engraftment to the CCl(4)-injured liver than the normoxic cells. Hypoxic treatment of BMCs may have merits in decreasing apoptosis of those cells as well as in enhancing cellular migration to SDF-1alpha, the chemokine which binds to BMCs expressed CXCR4 and to the injured tissue, such as CCl(4) damaged liver.
Volume 33(1)
Pages 31-5
Published 2009-1-1
DOI 10.1016/j.cellbi.2008.10.002
PII S1065-6995(08)00593-3
PMID 18992833
MeSH Animals Apoptosis Bone Marrow Cells / drug effects Bone Marrow Cells / physiology* Bone Marrow Transplantation Carbon Tetrachloride Poisoning / metabolism Cell Hypoxia Cell Movement / physiology* Chemokine CXCL12 / metabolism Female Flow Cytometry Liver / drug effects* Liver / enzymology Mice Mice, Inbred C57BL Mice, Transgenic Receptors, CXCR4 / drug effects Receptors, CXCR4 / metabolism
IF 2.127
Times Cited 5
Mice C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb