RRC ID 60019
Author Kobayashi Y, Shimazu T, Murata K, Itou T, Suzuki Y.
Title An endogenous adeno-associated virus element in elephants.
Journal Virus Res
Abstract An endogenous viral element derived from adeno-associated virus containing a nearly intact open reading frame (ORF) of the rep gene (enAAV-rep) has been identified in the genomes of various mammals including degu and African elephant. Particularly, in degu, mRNA expression of enAAV-rep has been observed specifically in the liver. Here we newly identified enAAV-rep in Asian elephant and rock hyrax, both of which are afrotherians. The enAAV-rep of African and Asian elephants appeared to be orthologous and originated from an integration event of the entire genome of AAV into the ancestral genome of elephants more than 6 million years ago, whereas that of rock hyrax appeared to have originated independently. Negative selection operating at the amino acid sequence level was detected for the ORF of enAAV-rep in elephants. As in degu, mRNA expression of enAAV-rep was specifically observed in the liver in Asian elephant. Integrations of enAAV-rep appeared to have occurred independently on the evolutionary lineages of elephants and degu, suggesting that the AAV Rep protein has been co-opted repeatedly in the mammalian liver.
Volume 262
Pages 10-14
Published 2019-3-1
DOI 10.1016/j.virusres.2018.04.015
PII S0168-1702(18)30160-6
PMID 29702129
MeSH Animals Asia Cell Line DNA-Binding Proteins / genetics Dependovirus / genetics* Elephants / genetics Elephants / virology* Evolution, Molecular Genome* Liver / virology Open Reading Frames / genetics Phylogeny Viral Proteins / genetics* Virus Integration*
IF 2.736
Times Cited 2
Human and Animal Cells LACF-NaNaII(RCB2320)