論文 - 詳細
| RRC ID | 60032 |
|---|---|
| 著者 | Abdelfattah AS, Kawashima T, Singh A, Novak O, Liu H, Shuai Y, Huang YC, Campagnola L, Seeman SC, Yu J, Zheng J, Grimm JB, Patel R, Friedrich J, Mensh BD, Paninski L, Macklin JJ, Murphy GJ, Podgorski K, Lin BJ, Chen TW, Turner GC, Liu Z, Koyama M, Svoboda K, Ahrens MB, Lavis LD, Schreiter ER. |
| タイトル | Bright and photostable chemigenetic indicators for extended in vivo voltage imaging. |
| ジャーナル | Science |
| Abstract |
Genetically encoded voltage indicators (GEVIs) enable monitoring of neuronal activity at high spatial and temporal resolution. However, the utility of existing GEVIs has been limited by the brightness and photostability of fluorescent proteins and rhodopsins. We engineered a GEVI, called Voltron, that uses bright and photostable synthetic dyes instead of protein-based fluorophores, thereby extending the number of neurons imaged simultaneously in vivo by a factor of 10 and enabling imaging for significantly longer durations relative to existing GEVIs. We used Voltron for in vivo voltage imaging in mice, zebrafish, and fruit flies. In the mouse cortex, Voltron allowed single-trial recording of spikes and subthreshold voltage signals from dozens of neurons simultaneously over a 15-minute period of continuous imaging. In larval zebrafish, Voltron enabled the precise correlation of spike timing with behavior. |
| 巻・号 | 365(6454) |
| ページ | 699-704 |
| 公開日 | 2019-8-16 |
| DOI | 10.1126/science.aav6416 |
| PII | science.aav6416 |
| PMID | 31371562 |
| MeSH | Animals Behavior, Animal Fluorescence Fluorescence Resonance Energy Transfer Genetic Engineering Larva Luminescent Proteins / chemistry Luminescent Proteins / genetics Mesencephalon / cytology Mesencephalon / physiology Mice Monitoring, Physiologic / methods* Neuroimaging / methods* Neurons / physiology* Optogenetics Protein Domains Rhodopsins, Microbial / chemistry Rhodopsins, Microbial / genetics Swimming Voltage-Sensitive Dye Imaging / methods* Zebrafish |
| IF | 41.063 |
| 引用数 | 46 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 270 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(vglut2a:Gal4) |