RRC ID 60052
Author Nimura T, Itoh T, Hagio H, Hayashi T, Di Donato V, Takeuchi M, Itoh T, Inoguchi F, Sato Y, Yamamoto N, Katsuyama Y, Del Bene F, Shimizu T, Hibi M.
Title Role of Reelin in cell positioning in the cerebellum and the cerebellum-like structure in zebrafish.
Journal Dev Biol
Abstract The cerebellum and the cerebellum-like structure in the mesencephalic tectum in zebrafish contain multiple cell types, including principal cells (i.e., Purkinje cells and type I neurons) and granule cells, that form neural circuits in which the principal cells receive and integrate inputs from granule cells and other neurons. It is largely unknown how these cells are positioned and how neural circuits form. While Reelin signaling is known to play an important role in cell positioning in the mammalian brain, its role in the formation of other vertebrate brains remains elusive. Here we found that zebrafish with mutations in Reelin or in the Reelin-signaling molecules Vldlr or Dab1a exhibited ectopic Purkinje cells, eurydendroid cells (projection neurons), and Bergmann glial cells in the cerebellum, and ectopic type I neurons in the tectum. The ectopic Purkinje cells and type I neurons received aberrant afferent fibers in these mutants. In wild-type zebrafish, reelin transcripts were detected in the internal granule cell layer, while Reelin protein was localized to the superficial layer of the cerebellum and the tectum. Laser ablation of the granule cell axons perturbed the localization of Reelin, and the mutation of both kif5aa and kif5ba, which encode major kinesin I components in the granule cells, disrupted the elongation of granule cell axons and the Reelin distribution. Our findings suggest that in zebrafish, (1) Reelin is transported from the granule cell soma to the superficial layer by axonal transport; (2) Reelin controls the migration of neurons and glial cells from the ventricular zone; and (3) Purkinje cells and type I neurons attract afferent axons during the formation of the cerebellum and the cerebellum-like structure.
Volume 455(2)
Pages 393-408
Published 2019-11-15
DOI 10.1016/j.ydbio.2019.07.010
PII S0012-1606(19)30049-1
PMID 31323192
MeSH Animals CRISPR-Cas Systems Cell Adhesion Molecules, Neuronal / genetics Cell Adhesion Molecules, Neuronal / physiology* Cell Movement Cerebellum / cytology Cerebellum / embryology* Extracellular Matrix Proteins / genetics Extracellular Matrix Proteins / physiology* Kinesins / metabolism Mutation Nerve Tissue Proteins / genetics Nerve Tissue Proteins / physiology* Purkinje Cells / cytology Reelin Protein Serine Endopeptidases / genetics Serine Endopeptidases / physiology* Signal Transduction Zebrafish / anatomy & histology Zebrafish / embryology* Zebrafish Proteins / genetics Zebrafish Proteins / physiology*
IF 2.936
Times Cited 1
Zebrafish aldoca:gap43-Venus hspGFFDMC90A hspGFFDMC28C hspzGFFgDMC156A SAGFF(LF)251A UAS:GFP