| RRC ID |
60085
|
| 著者 |
Satou C, Sugioka T, Uemura Y, Shimazaki T, Zmarz P, Kimura Y, Higashijima SI.
|
| タイトル |
Functional Diversity of Glycinergic Commissural Inhibitory Neurons in Larval Zebrafish.
|
| ジャーナル |
Cell Rep
|
| Abstract |
Commissural inhibitory neurons in the spinal cord of aquatic vertebrates coordinate left-right body alternation during swimming. Their developmental origin, however, has been elusive. We investigate this by comparing the anatomy and function of two commissural inhibitory neuron types, dI6dmrt3a and V0d, derived from the pd6 and p0 progenitor domains, respectively. We find that both of these commissural neuron types have monosynaptic, inhibitory connections to neuronal populations active during fictive swimming, supporting their role in providing inhibition to the contralateral side. V0d neurons tend to fire during faster and stronger movements, while dI6dmrt3a neurons tend to fire more consistently during normal fictive swimming. Ablation of dI6dmrt3a neurons leads to an impairment of left-right alternating activity through abnormal co-activation of ventral root neurons on both sides of the spinal cord. Our results suggest that dI6dmrt3a and V0d commissural inhibitory neurons synergistically provide inhibition to the opposite side across different swimming behaviors.
|
| 巻・号 |
30(9)
|
| ページ |
3036-3050.e4
|
| 公開日 |
2020-3-3
|
| DOI |
10.1016/j.celrep.2020.02.015
|
| PII |
S2211-1247(20)30169-8
|
| PMID |
32130905
|
| MeSH |
Animals
Gene Deletion
Glycine / metabolism*
Green Fluorescent Proteins / metabolism
Inhibitory Postsynaptic Potentials
Larva
Locomotion
Neural Inhibition / physiology*
Neurons / physiology*
Phenotype
Spinal Cord / physiology
Swimming
Transcription Factors / metabolism
Zebrafish / physiology*
Zebrafish Proteins / metabolism
|
| IF |
7.815
|
| 引用数 |
0
|
| リソース情報 |
| ゼブラフィッシュ |
Tg(dmrt3a-hs:Gal4)
Tg(dmrt3a-hs:GFP)
Tg(drmt3a-hs:Cre)
Tg(dbx1b:Cre)
Tg(glyt2-hs:lRl-GFP)
Tg(glyt2:Gal4)
Tg(UAS:GFP)
Tg(UAS:ChRWR)
Tg(glyt2:lRl-Gal4)
Tg(dmrt3a-hs:lRl-DTA)
Tg(hoxa9a-3’enhancer:Cre) |
