論文 - 詳細
| RRC ID | 60131 |
|---|---|
| 著者 | Sone E, Noshiro D, Ikebuchi Y, Nakagawa M, Khan M, Tamura Y, Ikeda M, Oki M, Murali R, Fujimori T, Yoda T, Honma M, Suzuki H, Ando T, Aoki K. |
| タイトル | The induction of RANKL molecule clustering could stimulate early osteoblast differentiation. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
We recently found that the membrane-bound receptor activator of NF-κB ligand (RANKL) on osteoblasts works as a receptor to stimulate osteoblast differentiation, however, the reason why the RANKL-binding molecules stimulate osteoblast differentiation has not been well clarified. Since the induction of cell-surface receptor clustering is known to lead to cell activation, we hypothesized that the induction of membrane-RANKL clustering on osteoblasts might stimulate osteoblast differentiation. Immunoblotting showed that the amount of RANKL on the membrane was increased by the RANKL-binding peptide OP3-4, but not by osteoprotegerin (OPG), the other RANKL-binding molecule, in Gfp-Rankl-transfected ST2 cells. Observation under a high-speed atomic force microscope (HS-AFM) revealed that RANKL molecules have the ability to form clusters. The induction of membrane-RANKL-OPG-Fc complex clustering by the addition of IgM in Gfp-Rankl-transfected ST2 cells could enhance the expression of early markers of osteoblast differentiation to the same extent as OP3-4, while OPG-Fc alone could not. These results suggest that the clustering-formation of membrane-RANKL on osteoblasts could stimulate early osteoblast differentiation. |
| 巻・号 | 509(2) |
| ページ | 435-440 |
| 公開日 | 2019-2-5 |
| DOI | 10.1016/j.bbrc.2018.12.093 |
| PII | S0006-291X(18)32742-6 |
| PMID | 30594398 |
| MeSH | Animals Binding Sites Cell Differentiation / drug effects* Cell Line Cell Membrane / drug effects Cell Membrane / metabolism Cell Membrane / ultrastructure Gene Expression Regulation Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Immunoglobulin Fc Fragments / genetics Immunoglobulin Fc Fragments / metabolism Immunoglobulin M / genetics Immunoglobulin M / metabolism Mice Microscopy, Atomic Force Models, Molecular Oligopeptides / chemistry Oligopeptides / metabolism Oligopeptides / pharmacology* Osteoblasts / drug effects* Osteoblasts / metabolism Osteoblasts / ultrastructure Osteoprotegerin / genetics Osteoprotegerin / metabolism Peptidomimetics / chemistry Peptidomimetics / metabolism Peptidomimetics / pharmacology* Protein Binding RANK Ligand / genetics* RANK Ligand / metabolism Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Signal Transduction Time Factors |
| IF | 2.705 |
| 引用数 | 3 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ST2(RCB0224) |